論文 - 詳細
| RRC ID | 45896 |
|---|---|
| 著者 | Yochem J, Lažetić V, Bell L, Chen L, Fay D. |
| タイトル | C. elegans NIMA-related kinases NEKL-2 and NEKL-3 are required for the completion of molting. |
| ジャーナル | Dev Biol |
| Abstract |
Caenorhabditis elegans molting is a process during which the apical extracellular matrix of the epidermis, the cuticle, is remodeled through a process of degradation and re-synthesis. Using a genetic approach, we identified nekl-3 as essential for the completion of molting. NEKL-3 is highly similar to the mammalian NEK kinase family members NEK6 and NEK7. Animals homozygous for a hypomorphic mutation in nekl-3, sv3, had a novel molting defect in which the central body region, but not the head or tail, was unable to shed the old cuticle. In contrast, a null mutation in nekl-3, gk506, led to complete enclosure within the old cuticle. nekl-2, which is most similar to mammalian NEK8, was also essential for molting. Mosaic analyses demonstrated that NEKL-2 and NEKL-3 were specifically required within the large epidermal syncytium, hyp7, to facilitate molting. Consistent with this, NEKL-2 and NEKL-3 were expressed at the apical surface of hyp7 where they localized to small spheres or tubular structures. Inhibition of nekl-2, but not nekl-3, led to the mislocalization of LRP-1/megalin, a cell surface receptor for low-density lipoprotein (LDL)-binding proteins. In addition, nekl-2 inhibition led to the mislocalization of several other endosome-associated proteins. Notably, LRP-1 acts within hyp7 to facilitate completion of molting, suggesting at least one mechanism by which NEKL-2 may influence molting. Notably, our studies failed to reveal a requirement for NEKL-2 or NEKL-3 in cell division, a function reported for several mammalian NEKs including NEK6 and NEK7. Our findings provide the first genetic and in vivo evidence for a role of NEK family members in endocytosis, which may be evolutionarily conserved. |
| 巻・号 | 398(2) |
| ページ | 255-66 |
| 公開日 | 2015-2-15 |
| DOI | 10.1016/j.ydbio.2014.12.008 |
| PII | S0012-1606(14)00634-4 |
| PMID | 25523392 |
| PMC | PMC4314388 |
| MeSH | Alleles Amino Acid Sequence Animals Biomarkers / metabolism Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Polarity Endocytosis Endosomes / metabolism Fluorescence Genes, Helminth Genes, Reporter Green Fluorescent Proteins / metabolism Molecular Sequence Data Molting* Mosaicism Mutation / genetics NIMA-Related Kinases Phenotype Phylogeny Protein Kinases / chemistry Protein Kinases / genetics Protein Kinases / metabolism* Sequence Alignment Subcutaneous Tissue / embryology Subcutaneous Tissue / metabolism |
| IF | 2.896 |
| 引用数 | 7 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm290 |