RRC ID 45964
Author Erkut C, Vasilj A, Boland S, Habermann B, Shevchenko A, Kurzchalia TV.
Title Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
Journal PLoS ONE
Abstract Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poorly understood. We recently showed that Caenorhabditis elegans dauer larva, an arrested stage specialized for survival in adverse conditions, is resistant to severe desiccation. However, this requires a preconditioning step at a mild desiccative environment to prepare the organism for harsher desiccation conditions. A systems approach was used to identify factors that are activated during this preconditioning. Using microarray analysis, proteomics, and bioinformatics, genes, proteins, and biochemical pathways that are upregulated during this process were identified. These pathways were validated via reverse genetics by testing the desiccation tolerances of mutants. These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons. This leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway. Remarkably, this response is specific and involves a small number of functional pathways, which represent the generic toolkit for anhydrobiosis in plants and animals.
Volume 8(12)
Pages e82473
Published 2013
DOI 10.1371/journal.pone.0082473
PII PONE-D-13-32134
PMID 24324795
PMC PMC3853187
MeSH Adaptation, Biological Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Cluster Analysis Cytoplasm / metabolism Dehydration / genetics* Dehydration / metabolism* Desiccation Fatty Acids, Unsaturated / metabolism Gene Expression Profiling Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism Larva / genetics* Larva / metabolism* Metabolic Networks and Pathways Neurons / physiology Polyamines / metabolism Protein Biosynthesis Protein Processing, Post-Translational Proteome Proteomics Reactive Oxygen Species / metabolism Sensation / physiology Stress, Physiological Transcriptome
IF 2.766
Times Cited 24
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm420 tm331 tm776 tm1146 tm3841 tm3809 tm918 tm951 tm5012 tm2318 tm1266 tm2895 tm2535 tm1990 tm3813