論文 - 詳細
| RRC ID | 45964 |
|---|---|
| 著者 | Erkut C, Vasilj A, Boland S, Habermann B, Shevchenko A, Kurzchalia TV. |
| タイトル | Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation. |
| ジャーナル | PLoS One |
| Abstract |
Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poorly understood. We recently showed that Caenorhabditis elegans dauer larva, an arrested stage specialized for survival in adverse conditions, is resistant to severe desiccation. However, this requires a preconditioning step at a mild desiccative environment to prepare the organism for harsher desiccation conditions. A systems approach was used to identify factors that are activated during this preconditioning. Using microarray analysis, proteomics, and bioinformatics, genes, proteins, and biochemical pathways that are upregulated during this process were identified. These pathways were validated via reverse genetics by testing the desiccation tolerances of mutants. These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons. This leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway. Remarkably, this response is specific and involves a small number of functional pathways, which represent the generic toolkit for anhydrobiosis in plants and animals. |
| 巻・号 | 8(12) |
| ページ | e82473 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0082473 |
| PII | PONE-D-13-32134 |
| PMID | 24324795 |
| PMC | PMC3853187 |
| MeSH | Adaptation, Biological Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Cluster Analysis Cytoplasm / metabolism Dehydration / genetics* Dehydration / metabolism* Desiccation Fatty Acids, Unsaturated / metabolism Gene Expression Profiling Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism Larva / genetics* Larva / metabolism* Metabolic Networks and Pathways Neurons / physiology Polyamines / metabolism Protein Biosynthesis Protein Processing, Post-Translational Proteome Proteomics Reactive Oxygen Species / metabolism Sensation / physiology Stress, Physiological Transcriptome |
| IF | 2.74 |
| 引用数 | 45 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm420 tm331 tm776 tm1146 tm3841 tm3809 tm918 tm951 tm5012 tm2318 tm1266 tm2895 tm2535 tm1990 tm3813 |