Reference - Detail
| RRC ID | 46023 |
|---|---|
| Author | Baudrimont A, Penkner A, Woglar A, Mamnun YM, Hulek M, Struck C, Schnabel R, Loidl J, Jantsch V. |
| Title | A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans. |
| Journal | PLoS One |
| Abstract |
The cohesin complex is required for the cohesion of sister chromatids and for correct segregation during mitosis and meiosis. Crossover recombination, together with cohesion, is essential for the disjunction of homologous chromosomes during the first meiotic division. Cohesin has been implicated in facilitating recombinational repair of DNA lesions via the sister chromatid. Here, we made use of a new temperature-sensitive mutation in the Caenorhabditis elegans SMC-3 protein to study the role of cohesin in the repair of DNA double-strand breaks (DSBs) and hence in meiotic crossing over. We report that attenuation of cohesin was associated with extensive SPO-11-dependent chromosome fragmentation, which is representative of unrepaired DSBs. We also found that attenuated cohesin likely increased the number of DSBs and eliminated the need of MRE-11 and RAD-50 for DSB formation in C. elegans, which suggests a role for the MRN complex in making cohesin-loaded chromatin susceptible to meiotic DSBs. Notably, in spite of largely intact sister chromatid cohesion, backup DSB repair via the sister chromatid was mostly impaired. We also found that weakened cohesins affected mitotic repair of DSBs by homologous recombination, whereas NHEJ repair was not affected. Our data suggest that recombinational DNA repair makes higher demands on cohesins than does chromosome segregation. |
| Volume | 6(9) |
| Pages | e24799 |
| Published | 2011-1-1 |
| DOI | 10.1371/journal.pone.0024799 |
| PII | PONE-D-11-10788 |
| PMID | 21957461 |
| PMC | PMC3177864 |
| MeSH | Alleles* Amino Acid Sequence Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / chemistry Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Chromatids / genetics Chromatin / genetics Chromatin / metabolism Chromosomal Proteins, Non-Histone / chemistry Chromosomal Proteins, Non-Histone / genetics* Chromosomal Proteins, Non-Histone / metabolism Chromosome Pairing / genetics Cohesins DNA Breaks, Double-Stranded DNA Repair / genetics Homologous Recombination / genetics* Humans Kinetics Male Mice Molecular Sequence Data Mutation Temperature* Testis / growth & development |
| IF | 2.74 |
| Times Cited | 12 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2553 |