RRC ID 46186
Author Sato M, Konuma R, Sato K, Tomura K, Sato K.
Title Fertilization-induced K63-linked ubiquitylation mediates clearance of maternal membrane proteins.
Journal Development
Abstract In Caenorhabditis elegans, fertilization triggers endocytosis and rapid turnover of maternal surface membrane proteins in lysosomes, although the precise mechanism of this inducible endocytosis is unknown. We found that high levels of K63-linked ubiquitin chains transiently accumulated on endosomes upon fertilization. Endocytosis and the endosomal accumulation of ubiquitin were both regulated downstream of the anaphase-promoting complex, which drives the oocyte's meiotic cell cycle after fertilization. The clearance of maternal membrane proteins and the accumulation of K63-linked ubiquitin on endosomes depended on UBC-13 and UEV-1, which function as an E2 complex that specifically mediates chain elongation of K63-linked polyubiquitin. CAV-1-GFP, an endocytic cargo protein, was modified with K63-linked polyubiquitin in a UBC-13/UEV-1-dependent manner. In ubc-13 or uev-1 mutants, CAV-1-GFP and other membrane proteins were internalized from the plasma membrane normally after fertilization. However, they were not efficiently targeted to the multivesicular body (MVB) pathway but recycled to the cell surface. Our results suggest that UBC-13-dependent K63-linked ubiquitylation is required for proper MVB sorting rather than for internalization. These results also demonstrate a developmentally controlled function of K63-linked ubiquitylation.
Volume 141(6)
Pages 1324-31
Published 2014-3
DOI 10.1242/dev.103044
PII 141/6/1324
PMID 24595290
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caveolins / genetics Caveolins / metabolism Endocytosis Female Fertilization Genes, Helminth Male Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Oocytes / metabolism Protein Transport Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism Ubiquitination Zygote / metabolism
IF 5.763
Times Cited 5
WOS Category DEVELOPMENTAL BIOLOGY
Resource
C.elegans tm3546