論文 - 詳細
| RRC ID | 46186 |
|---|---|
| 著者 | Sato M, Konuma R, Sato K, Tomura K, Sato K. |
| タイトル | Fertilization-induced K63-linked ubiquitylation mediates clearance of maternal membrane proteins. |
| ジャーナル | Development |
| Abstract |
In Caenorhabditis elegans, fertilization triggers endocytosis and rapid turnover of maternal surface membrane proteins in lysosomes, although the precise mechanism of this inducible endocytosis is unknown. We found that high levels of K63-linked ubiquitin chains transiently accumulated on endosomes upon fertilization. Endocytosis and the endosomal accumulation of ubiquitin were both regulated downstream of the anaphase-promoting complex, which drives the oocyte's meiotic cell cycle after fertilization. The clearance of maternal membrane proteins and the accumulation of K63-linked ubiquitin on endosomes depended on UBC-13 and UEV-1, which function as an E2 complex that specifically mediates chain elongation of K63-linked polyubiquitin. CAV-1-GFP, an endocytic cargo protein, was modified with K63-linked polyubiquitin in a UBC-13/UEV-1-dependent manner. In ubc-13 or uev-1 mutants, CAV-1-GFP and other membrane proteins were internalized from the plasma membrane normally after fertilization. However, they were not efficiently targeted to the multivesicular body (MVB) pathway but recycled to the cell surface. Our results suggest that UBC-13-dependent K63-linked ubiquitylation is required for proper MVB sorting rather than for internalization. These results also demonstrate a developmentally controlled function of K63-linked ubiquitylation. |
| 巻・号 | 141(6) |
| ページ | 1324-31 |
| 公開日 | 2014-3-1 |
| DOI | 10.1242/dev.103044 |
| PII | 141/6/1324 |
| PMID | 24595290 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caveolins / genetics Caveolins / metabolism Endocytosis Female Fertilization Genes, Helminth Male Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Oocytes / metabolism Protein Transport Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism Ubiquitination Zygote / metabolism |
| IF | 5.611 |
| 引用数 | 13 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3546 |