論文 - 詳細
| RRC ID | 46209 |
|---|---|
| 著者 | Swiatkowski P, Sesti F. |
| タイトル | Delayed pharyngeal repolarization promotes abnormal calcium buildup in aging muscle. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
In the pharynx of Caenorhabditis elegans, the accessory subunit MPS-4, homolog to human KCNE1, forms a complex with K(+) channel EXP-2 that terminates the action potential. An aspartate residue critical for KCNE1 function, asp76, is conserved in MPS-4 (asp74). Here, we studied the effects of D74N-MPS-4 on the aging pharynx. Electrophysiological studies showed that D74N delays pharyngeal repolarization. Pharynxes of transgenic worms expressing D74N exhibited higher levels of intracellular calcium compared to normal pharynxes. Accordingly, loss of pharyngeal function was accelerated in aging D74N worms. The pharyngeal action potential resembles the action potential that controls the mechanical activity of human left ventricle. Hence, these findings argue that the hearts of patients affected by delayed repolarization, a condition known as long QT syndrome, may experience dysregulated calcium homeostasis. |
| 巻・号 | 433(3) |
| ページ | 354-7 |
| 公開日 | 2013-4-12 |
| DOI | 10.1016/j.bbrc.2013.03.011 |
| PII | S0006-291X(13)00409-9 |
| PMID | 23510998 |
| MeSH | Action Potentials / physiology* Aging / metabolism* Animals Aspartic Acid / chemistry Aspartic Acid / genetics Aspartic Acid / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Calcium / metabolism* Heart Ventricles / metabolism Heart Ventricles / pathology Humans Long QT Syndrome / metabolism Long QT Syndrome / pathology Models, Biological Mutation Myocardium / metabolism Myocardium / pathology Pharynx / metabolism* Potassium Channels / genetics Potassium Channels / metabolism Potassium Channels, Voltage-Gated / chemistry Potassium Channels, Voltage-Gated / genetics Potassium Channels, Voltage-Gated / metabolism* Protein Binding Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / metabolism Protein Subunits / chemistry Protein Subunits / genetics Protein Subunits / metabolism* Sequence Homology, Amino Acid |
| IF | 2.985 |
| 引用数 | 2 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 線虫 | tm2596 |