Reference - Detail
| RRC ID | 46247 |
|---|---|
| Author | Zhang P, Zhang H. |
| Title | Autophagy modulates miRNA-mediated gene silencing and selectively degrades AIN-1/GW182 in C. elegans. |
| Journal | EMBO Rep |
| Abstract |
MicroRNAs (miRNAs) post-transcriptionally repress gene expression via the miRNA-induced silencing complex (miRISC), which includes miRNA, Argonaute and a GW182 family member. Here we show that in Caenorhabditis elegans, miRNA-mediated gene silencing is modulated by macroautophagy, a lysosome-mediated degradation process. Loss of autophagy activity suppresses developmental defects caused by partially impaired silencing of miRNA targets including the let-7 family and lsy-6. The C. elegans GW182 homolog AIN-1 is itself selectively degraded by autophagy and colocalizes with the p62 homolog SQST-1 in autophagy mutants. Thus, autophagy activity modulates miRNA-mediated gene silencing and degrades a core miRISC component. |
| Volume | 14(6) |
| Pages | 568-76 |
| Published | 2013-6-1 |
| DOI | 10.1038/embor.2013.53 |
| PII | embor201353 |
| PMID | 23619095 |
| PMC | PMC3674441 |
| MeSH | Animals Autophagy* Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carrier Proteins / genetics* Carrier Proteins / metabolism Gene Expression Regulation, Developmental Larva / cytology Larva / genetics Larva / growth & development MicroRNAs / genetics* Mutation Proteolysis RNA Interference* RNA-Induced Silencing Complex / genetics ras Proteins / genetics ras Proteins / metabolism |
| IF | 7.497 |
| Times Cited | 35 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| C.elegans | tm3425 tm2508 |