RRC ID 46313
Author Oikonomou G, Perens EA, Lu Y, Shaham S.
Title Some, but not all, retromer components promote morphogenesis of C. elegans sensory compartments.
Journal Dev Biol
Abstract The endings of sensory receptor cells often lie within specialized compartments formed by glial cells. The main sensory organ of Caenorhabditis elegans, the amphid, provides a powerful setting for studying glial compartment morphogenesis. Our previous studies showed that amphid compartment size is controlled by opposing activities of the Nemo-like kinase LIT-1, which promotes compartment expansion, and the Patched-related protein DAF-6, which restricts compartment growth. From a genetic screen for mutations able to suppress the bloated sensory compartments of daf-6 mutants, we identified an allele of the sorting nexin gene snx-1. SNX-1 protein is a component of the retromer, a protein complex that facilitates recycling of transmembrane proteins from the endosome to the Golgi network. We find that snx-1 functions cell autonomously within glia to promote sensory compartment growth, and that SNX-1 protein is enriched near the surface of the sensory compartment. snx-1 interacts genetically with lit-1 and another regulator of compartment size, the Dispatched-related gene che-14. Mutations in snx-3 and vps-29, also retromer genes, can suppress daf-6 defects. Surprisingly, however, remaining retromer components seem not to be involved. Our results suggest that a novel assembly of retromer components is important for determining sensory compartment dimensions.
Volume 362(1)
Pages 42-9
Published 2012-2-1
DOI 10.1016/j.ydbio.2011.11.009
PII S0012-1606(11)01378-9
PMID 22138055
PMC PMC3254776
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Membrane Proteins / metabolism Microscopy, Electron, Transmission Microscopy, Fluorescence Morphogenesis / physiology* Multiprotein Complexes / genetics* Multiprotein Complexes / physiology Mutation / genetics Neuroglia / physiology* Plasmids / genetics Protein Serine-Threonine Kinases / metabolism Sensory Receptor Cells / physiology* Sorting Nexins / genetics* Sorting Nexins / metabolism Sorting Nexins / physiology
IF 2.896
Times Cited 8
C.elegans tm847 tm1595 tm3790 tm2423 tm1523 tm1320