論文 - 詳細
| RRC ID | 46390 |
|---|---|
| 著者 | Kumsta C, Hansen M. |
| タイトル | C. elegans rrf-1 mutations maintain RNAi efficiency in the soma in addition to the germline. |
| ジャーナル | PLoS One |
| Abstract |
Gene inactivation through RNA interference (RNAi) has proven to be a valuable tool for studying gene function in C. elegans. When combined with tissue-specific gene inactivation methods, RNAi has the potential to shed light on the function of a gene in distinct tissues. In this study we characterized C. elegans rrf-1 mutants to determine their ability to process RNAi in various tissues. These mutants have been widely used in RNAi studies to assess the function of genes specifically in the C. elegans germline. Upon closer analysis, we found that two rrf-1 mutants carrying different loss-of-function alleles were capable of processing RNAi targeting several somatically expressed genes. Specifically, we observed that the intestine was able to process RNAi triggers efficiently, whereas cells in the hypodermis showed partial susceptibility to RNAi in rrf-1 mutants. Other somatic tissues in rrf-1 mutants, such as the muscles and the somatic gonad, appeared resistant to RNAi. In addition to these observations, we found that the rrf-1(pk1417) mutation induced the expression of several transgenic arrays, including the FOXO transcription factor DAF-16. Unexpectedly, rrf-1(pk1417) mutants showed increased endogenous expression of the DAF-16 target gene sod-3; however, the lifespan and thermo-tolerance of rrf-1(pk1417) mutants were similar to those of wild-type animals. In sum, these data show that rrf-1 mutants display several phenotypes not previously appreciated, including broader tissue-specific RNAi-processing capabilities, and our results underscore the need for careful characterization of tissue-specific RNAi tools. |
| 巻・号 | 7(5) |
| ページ | e35428 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0035428 |
| PII | PONE-D-11-22140 |
| PMID | 22574120 |
| PMC | PMC3344830 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Female Forkhead Transcription Factors Gene Expression Regulation / genetics Genes, Reporter / genetics Intestinal Mucosa / metabolism Male Mutation* Organ Specificity Ovum / growth & development Ovum / metabolism* RNA Interference* RNA-Dependent RNA Polymerase / genetics* RNA-Dependent RNA Polymerase / metabolism* Spermatozoa / growth & development Spermatozoa / metabolism* Subcutaneous Tissue / metabolism Superoxide Dismutase / deficiency Superoxide Dismutase / genetics Superoxide Dismutase / metabolism Transcription Factors / deficiency Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 2.74 |
| 引用数 | 65 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1887 tm1716 tm1917 |