| RRC ID |
46405
|
| Author |
McNally KL, Fabritius AS, Ellefson ML, Flynn JR, Milan JA, McNally FJ.
|
| Title |
Kinesin-1 prevents capture of the oocyte meiotic spindle by the sperm aster.
|
| Journal |
Dev Cell
|
| Abstract |
Centrioles are lost during oogenesis and inherited from the sperm at fertilization. In the zygote, the centrioles recruit pericentriolar proteins from the egg to form a mature centrosome that nucleates a sperm aster. The sperm aster then captures the female pronucleus to join the maternal and paternal genomes. Because fertilization occurs before completion of female meiosis, some mechanism must prevent capture of the meiotic spindle by the sperm aster. Here we show that in wild-type Caenorhabditis elegans zygotes, maternal pericentriolar proteins are not recruited to the sperm centrioles until after completion of meiosis. Depletion of kinesin-1 heavy chain or its binding partner resulted in premature centrosome maturation during meiosis and growth of a sperm aster that could capture the oocyte meiotic spindle. Kinesin prevents recruitment of pericentriolar proteins by coating the sperm DNA and centrioles and thus prevents triploidy by a nonmotor mechanism.
|
| Volume |
22(4)
|
| Pages |
788-98
|
| Published |
2012-4-17
|
| DOI |
10.1016/j.devcel.2012.01.010
|
| PII |
S1534-5807(12)00044-5
|
| PMID |
22465668
|
| PMC |
PMC3606814
|
| MeSH |
Animals
Caenorhabditis elegans / embryology
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins
Cell Cycle Proteins
Cells, Cultured
Centrioles / metabolism*
Centrosome / physiology*
Embryo, Nonmammalian
Female
Fertilization
Fluorescent Antibody Technique
Kinesins / antagonists & inhibitors
Kinesins / genetics
Kinesins / metabolism*
Male
Meiosis / physiology*
Oocytes / cytology
Oocytes / physiology*
Oogenesis / physiology
Spermatozoa / cytology
Spermatozoa / physiology*
Spindle Apparatus / physiology
|
| IF |
10.092
|
| Times Cited |
14
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1489
tm1145 |
