論文 - 詳細
| RRC ID | 46450 |
|---|---|
| 著者 | Stavoe AK, Nelson JC, Martínez-Velázquez LA, Klein M, Samuel AD, Colón-Ramos DA. |
| タイトル | Synaptic vesicle clustering requires a distinct MIG-10/Lamellipodin isoform and ABI-1 downstream from Netrin. |
| ジャーナル | Genes Dev |
| Abstract |
The chemotrophic factor Netrin can simultaneously instruct different neurodevelopmental programs in individual neurons in vivo. How neurons correctly interpret the Netrin signal and undergo the appropriate neurodevelopmental response is not understood. Here we identify MIG-10 isoforms as critical determinants of individual cellular responses to Netrin. We determined that distinct MIG-10 isoforms, varying only in their N-terminal motifs, can localize to specific subcellular domains and are differentially required for discrete neurodevelopmental processes in vivo. We identified MIG-10B as an isoform uniquely capable of localizing to presynaptic regions and instructing synaptic vesicle clustering in response to Netrin. MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. We identified a motif in the MIG-10B N-terminal domain that is required for its function and localization to presynaptic sites. With this motif, we engineered a dominant-negative MIG-10B construct that disrupts vesicle clustering and animal thermotaxis behavior when expressed in a single neuron in vivo. Our findings indicate that the unique N-terminal domains confer distinct MIG-10 isoforms with unique capabilities to localize to distinct subcellular compartments, organize the actin cytoskeleton at these sites, and instruct distinct Netrin-dependent neurodevelopmental programs. |
| 巻・号 | 26(19) |
| ページ | 2206-21 |
| 公開日 | 2012-10-1 |
| DOI | 10.1101/gad.193409.112 |
| PII | 26/19/2206 |
| PMID | 23028145 |
| PMC | PMC3465741 |
| MeSH | Actin Cytoskeleton / metabolism Animals Behavior, Animal / physiology Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Movement Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Gene Expression Profiling Interneurons / cytology Motor Neurons / cytology Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Netrins Protein Isoforms Protein Transport / genetics Synaptic Vesicles / genetics Synaptic Vesicles / metabolism* |
| IF | 9.527 |
| 引用数 | 25 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm494 tm2557 |