RRC ID 46451
Author Studencka M, Wesołowski R, Opitz L, Salinas-Riester G, Wisniewski JR, Jedrusik-Bode M.
Title Transcriptional repression of Hox genes by C. elegans HP1/HPL and H1/HIS-24.
Journal PLoS Genet
Abstract Elucidation of the biological role of linker histone (H1) and heterochromatin protein 1 (HP1) in mammals has been difficult owing to the existence of a least 11 distinct H1 and three HP1 subtypes in mice. Caenorhabditis elegans possesses two HP1 homologues (HPL-1 and HPL-2) and eight H1 variants. Remarkably, one of eight H1 variants, HIS-24, is important for C. elegans development. Therefore we decided to analyse in parallel the transcriptional profiles of HIS-24, HPL-1/-2 deficient animals, and their phenotype, since hpl-1, hpl-2, and his-24 deficient nematodes are viable. Global transcriptional analysis of the double and triple mutants revealed that HPL proteins and HIS-24 play gene-specific roles, rather than a general repressive function. We showed that HIS-24 acts synergistically with HPL to allow normal reproduction, somatic gonad development, and vulval cell fate decision. Furthermore, the hpl-2; his-24 double mutant animals displayed abnormal development of the male tail and ectopic expression of C. elegans HOM-C/Hox genes (egl-5 and mab-5), which are involved in the developmental patterning of male mating structures. We found that HPL-2 and the methylated form of HIS-24 specifically interact with the histone H3 K27 region in the trimethylated state, and HIS-24 associates with the egl-5 and mab-5 genes. Our results establish the interplay between HPL-1/-2 and HIS-24 proteins in the regulation of positional identity in C. elegans males.
Volume 8(9)
Pages e1002940
Published 2012-9-1
DOI 10.1371/journal.pgen.1002940
PII PGENETICS-D-12-00349
PMID 23028351
PMC PMC3441639
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Chromosomal Proteins, Non-Histone* / deficiency Chromosomal Proteins, Non-Histone* / genetics Female Gene Expression Regulation, Developmental Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism Histones / deficiency Histones / genetics* Histones / metabolism Homeodomain Proteins / metabolism Male Methylation Mutation Tail / growth & development Transcription Factors / metabolism Vulva / cytology Vulva / growth & development
IF 5.175
Times Cited 18
WOS Category GENETICS & HEREDITY
Resource
C.elegans tm1489 tm1624