RRC ID 46461
Author Vermezovic J, Stergiou L, Hengartner MO, d'Adda di Fagagna F.
Title Differential regulation of DNA damage response activation between somatic and germline cells in Caenorhabditis elegans.
Journal Cell Death Differ.
Abstract The germline of Caenorhabditis elegans is a well-established model for DNA damage response (DDR) studies. However, the molecular basis of the observed cell death resistance in the soma of these animals remains unknown. We established a set of techniques to study ionizing radiation-induced DNA damage generation and DDR activation in a whole intact worm. Our single-cell analyses reveal that, although germline and somatic cells show similar levels of inflicted DNA damage, somatic cells, differently from germline cells, do not activate the crucial apical DDR kinase ataxia-telengiectasia mutated (ATM). We also show that DDR signaling proteins are undetectable in all somatic cells and this is due to transcriptional repression. However, DNA repair genes are expressed and somatic cells retain the ability to efficiently repair DNA damage. Finally, we demonstrate that germline cells, when induced to transdifferentiate into somatic cells within the gonad, lose the ability to activate ATM. Overall, these observations provide a molecular mechanism for the known, but hitherto unexplained, resistance to DNA damage-induced cell death in C. elegans somatic cells. We propose that the observed lack of signaling and cell death but retention of DNA repair functions in the soma is a Caenorhabditis-specific evolutionary-selected strategy to cope with its lack of adult somatic stem cell pools and regenerative capacity.
Volume 19(11)
Pages 1847-55
Published 2012-11
DOI 10.1038/cdd.2012.69
PII cdd201269
PMID 22705849
PMC PMC3469062
MeSH Animals Apoptosis Ataxia Telangiectasia Mutated Proteins Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism DNA Damage DNA Repair* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Germ Cells / metabolism* Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism Radiation, Ionizing Signal Transduction Single-Cell Analysis Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism
IF 8.0
Times Cited 20
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1524 tm853 tm1724