Reference - Detail
RRC ID | 46506 |
---|---|
Author | Song MH, Liu Y, Anderson DE, Jahng WJ, O'Connell KF. |
Title | Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors. |
Journal | Dev Cell |
Abstract |
Centrioles play a crucial role in mitotic spindle assembly and duplicate precisely once per cell cycle. In worms, flies, and humans, centriole assembly is dependent upon a key regulatory kinase (ZYG-1/Sak/Plk4) and its downstream effectors SAS-5 and SAS-6. Here we report a role for protein phosphatase 2A (PP2A) in centriole duplication. We find that the PP2A catalytic subunit LET-92, the scaffolding subunit PAA-1, and the B55 regulatory subunit SUR-6 function together to positively regulate centriole assembly. In PP2A-SUR-6-depleted embryos, the levels of ZYG-1 and SAS-5 are reduced and the ZYG-1- and SAS-5-dependent recruitment of SAS-6 to the nascent centriole fails. We show that PP2A physically associates with SAS-5 in vivo and that inhibiting proteolysis can rescue SAS-5 levels and the centriole duplication defect of PP2A-depleted embryos. Together, our findings indicate that PP2A-SUR-6 promotes centriole assembly by protecting ZYG-1 and SAS-5 from degradation. |
Volume | 20(4) |
Pages | 563-71 |
Published | 2011-4-19 |
DOI | 10.1016/j.devcel.2011.03.007 |
PII | S1534-5807(11)00089-X |
PMID | 21497766 |
PMC | PMC3079880 |
MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Centrioles / genetics Centrioles / metabolism* Gene Duplication* Protein Phosphatase 2 / genetics Protein Phosphatase 2 / metabolism* Protein Subunits / genetics Protein Subunits / metabolism* |
IF | 10.092 |
Times Cited | 42 |
WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
Resource | |
C.elegans | tm395 tm655 |