論文 - 詳細
| RRC ID | 46518 |
|---|---|
| 著者 | Thivierge C, Makil N, Flamand M, Vasale JJ, Mello CC, Wohlschlegel J, Conte D Jr, Duchaine TF. |
| タイトル | Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi. |
| ジャーナル | Nat Struct Mol Biol |
| Abstract |
Endogenous RNA interference (endo-RNAi) pathways use a variety of mechanisms to generate siRNA and to mediate gene silencing. In Caenorhabditis elegans, DCR-1 is essential for competing RNAi pathways-the ERI endo-RNAi pathway and the exogenous RNAi pathway-to function. Here, we demonstrate that DCR-1 forms exclusive complexes in each pathway and further define the ERI-DCR-1 complex. We show that the tandem tudor protein ERI-5 potentiates ERI endo-RNAi by tethering an RNA-dependent RNA polymerase (RdRP) module to DCR-1. In the absence of ERI-5, the RdRP module is uncoupled from DCR-1. Notably, EKL-1, an ERI-5 paralog that specifies distinct RdRP modules in Dicer-independent endo-RNAi pathways, partially compensates for the loss of ERI-5 without interacting with DCR-1. Our results implicate tudor proteins in the recruitment of RdRP complexes to specific steps within DCR-1-dependent and DCR-1-independent endo-RNAi pathways. |
| 巻・号 | 19(1) |
| ページ | 90-7 |
| 公開日 | 2011-12-18 |
| DOI | 10.1038/nsmb.2186 |
| PII | nsmb.2186 |
| PMID | 22179787 |
| PMC | PMC3684169 |
| MeSH | Animals Blotting, Western Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Embryo, Nonmammalian / metabolism Immunoprecipitation Mutation Protein Binding RNA Interference* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism* RNA-Induced Silencing Complex / genetics RNA-Induced Silencing Complex / metabolism Recombinant Proteins / metabolism Ribonuclease III / genetics Ribonuclease III / metabolism* |
| IF | 11.98 |
| 引用数 | 27 |
| リソース情報 | |
| 線虫 | tm2528 tm1361 tm1329 |