Reference - Detail
| RRC ID | 46560 |
|---|---|
| Author | Adamo A, Collis SJ, Adelman CA, Silva N, Horejsi Z, Ward JD, Martinez-Perez E, Boulton SJ, La Volpe A. |
| Title | Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia. |
| Journal | Mol Cell |
| Abstract |
Fanconi anemia (FA) is a complex cancer susceptibility disorder associated with DNA repair defects and infertility, yet the precise function of the FA proteins in genome maintenance remains unclear. Here we report that C. elegans FANCD2 (fcd-2) is dispensable for normal meiotic recombination but is required in crossover defective mutants to prevent illegitimate repair of meiotic breaks by nonhomologous end joining (NHEJ). In mitotic cells, we show that DNA repair defects of C. elegans fcd-2 mutants and FA-deficient human cells are significantly suppressed by eliminating NHEJ. Moreover, NHEJ factors are inappropriately recruited to sites of replication stress in the absence of FANCD2. Our findings are consistent with the interpretation that FA results from the promiscuous action of NHEJ during DNA repair. We propose that a critical function of the FA pathway is to channel lesions into accurate, as opposed to error-prone, repair pathways. |
| Volume | 39(1) |
| Pages | 25-35 |
| Published | 2010-7-9 |
| DOI | 10.1016/j.molcel.2010.06.026 |
| PII | S1097-2765(10)00493-4 |
| PMID | 20598602 |
| MeSH | Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / metabolism Cross-Linking Reagents / metabolism Crossing Over, Genetic DNA Breaks, Double-Stranded DNA Repair / genetics* DNA Replication DNA-Activated Protein Kinase / metabolism Fanconi Anemia / genetics* Fanconi Anemia / pathology Fanconi Anemia Complementation Group D2 Protein / deficiency Fanconi Anemia Complementation Group D2 Protein / metabolism Humans Meiosis / genetics Mutation / genetics Rad51 Recombinase / metabolism Recombination, Genetic* Stress, Physiological |
| IF | 15.584 |
| Times Cited | 191 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1298 |