RRC ID 46563
著者 Alberti A, Michelet X, Djeddi A, Legouis R.
タイトル The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans.
ジャーナル Autophagy
Abstract Autophagy has an important function in degrading cytoplasmic components to maintain cellular homeostasis, but is also required during development. The formation of the autophagic vesicles requires the recruitment of the Atg8 ubiquitin-like proteins to the membrane of the nascent autophagosomes. Atg8 is a highly conserved gene which has been duplicated during metazoan evolution. In this report we have investigated, in the nematode C. elegans, the functions and localizations of the two Atg8p homologues LGG-2 and LGG-1. Phylogenetic analyses suggest that LGG-2 is more closely related to the human protein LC3 than LGG-1. LGG-1 but not LGG-2 is able to functionally complement the atg8 mutant yeast. The C-terminal glycine residue of LGG-2 is essential for post-translational modification and localization to the autophagosomes. During C. elegans development the two proteins share a similar expression pattern and localization but LGG-2 is more abundant in the neurons. Using genetic tools to either reduce or increase the autophagic flux we show that both LGG-2 and LGG-1 are addressed to the autophagosomal/lysosomal degradative system. We also demonstrate that the localization of both proteins is modified in several physiological processes when autophagy is induced, namely during diapause "dauer" larval formation, starvation and aging. Finally, we demonstrate that both LGG-2 and LGG-1 act synergistically and are involved in dauer formation and longevity of the worm.
巻・号 6(5)
ページ 622-33
公開日 2010-7-1
DOI 10.4161/auto.6.5.12252
PII 12252
PMID 20523114
MeSH Amino Acid Sequence Animals Autophagy* / genetics Autophagy-Related Protein 8 Family Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental Genes, Helminth / genetics Genome / genetics Glycine / metabolism Humans Larva / physiology Longevity / genetics Longevity / physiology* Lysosomes / metabolism Microtubule-Associated Proteins / genetics Molecular Sequence Data Mutation / genetics Phagosomes / metabolism* Protein Transport Saccharomyces cerevisiae Proteins / genetics Sequence Homology, Nucleic Acid
IF 9.77
引用数 45
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm3489