RRC ID 46582
Author Couteau F, Zetka M.
Title DNA damage during meiosis induces chromatin remodeling and synaptonemal complex disassembly.
Journal Dev. Cell
Abstract DNA damage to the germline genome must be accurately repaired to ensure transmission of intact genetic information to following generations. Meiosis presents challenges to the DNA damage response (DDR) because it universally requires changes to chromosome structure that can affect DNA repair outcomes. We report the existence of a meiotic DDR at chromosome axes that results in chromatin remodeling, synaptonemal complex disassembly, and axis separation in response to irradiation at late pachytene stages in C. elegans. The axis component HTP-3 is required for germline acquisition of H2AacK5, an axis-specific chromatin mark that is DNA damage responsive. Irradiated wild-types show reduction of H2AacK5 and axis separation that are dependent on the acetyltransferase MYS-1/TIP60. Restoration of H2AacK5 levels requires ATM-1 kinase and correlates with resynapsis. We propose that the meiotic DDR involves early chromatin remodeling at chromosome axes to dismantle structures promoting interhomolog recombination and facilitate efficient nonhomolog-based repair before pachytene exit.
Volume 20(3)
Pages 353-63
Published 2011-3-15
DOI 10.1016/j.devcel.2011.01.015
PII S1534-5807(11)00043-8
PMID 21397846
MeSH Animals Ataxia Telangiectasia Mutated Proteins Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromatin / genetics Chromatin / metabolism Chromatin Assembly and Disassembly* Chromosomes / metabolism Crossing Over, Genetic DNA Damage* DNA Repair Histones / genetics Histones / metabolism Meiosis / physiology* Phosphotransferases / genetics Phosphotransferases / metabolism Recombination, Genetic Synaptonemal Complex / metabolism*
IF 9.19
Times Cited 19
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm3655 tm1145