論文 - 詳細
| RRC ID | 46599 |
|---|---|
| 著者 | Guo P, Hu T, Zhang J, Jiang S, Wang X. |
| タイトル | Sequential action of Caenorhabditis elegans Rab GTPases regulates phagolysosome formation during apoptotic cell degradation. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Phagocytosis of apoptotic cells requires recognition of cell corpses followed by internalization and enclosure within plasma membrane-derived phagosomes. Phagosomes undergo maturation to generate phagolysosomes in which cell corpses are degraded; however, regulation of the maturation process is poorly understood. Here, we identified Rab GTPase 14, which regulates apoptotic cell degradation in Caenorhabditis elegans. rab-14 mutants accumulate many persistent cell corpses owing to defective cell corpse clearance. Loss of rab-14 function affects several steps of phagosome maturation including phagosomal acidification and phagolysosome formation. RAB-14 and UNC-108/RAB2 are recruited to phagosomes at a similar stage and function redundantly to regulate phagosome maturation. Three Rabs, RAB-14, UNC-108/RAB2, and RAB-7, act in sequential steps to control phagolysosome formation. RAB-14 and UNC-108 recruit lysosomes, whereas RAB-7 mediates fusion of lysosomes to phagosomes. Our data reveal the sequential action of Rab GTPases in regulating tethering, docking, and fusion of lysosomes to apoptotic cell-containing phagosomes. |
| 巻・号 | 107(42) |
| ページ | 18016-21 |
| 公開日 | 2010-10-19 |
| DOI | 10.1073/pnas.1008946107 |
| PII | 1008946107 |
| PMID | 20921409 |
| PMC | PMC2964220 |
| MeSH | Acids / metabolism Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Lysosomes / metabolism* Phagosomes / metabolism* rab GTP-Binding Proteins / metabolism* |
| IF | 9.412 |
| 引用数 | 50 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2095 |