論文 - 詳細
| RRC ID | 46636 |
|---|---|
| 著者 | Liu J, Ward A, Gao J, Dong Y, Nishio N, Inada H, Kang L, Yu Y, Ma D, Xu T, Mori I, Xie Z, Xu XZ. |
| タイトル | C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog. |
| ジャーナル | Nat Neurosci |
| Abstract |
The eyeless animal C. elegans is able to sense light and engages in phototaxis behavior that is mediated by photoreceptor cells. However, the molecular and cellular mechanisms underlying phototransduction in C. elegans remain largely unclear. By recording the photoreceptor neuron ASJ in wild-type and various mutant worms, we found that phototransduction in ASJ is a G protein-mediated process and requires membrane-associated guanylate cyclases, but not typical phosphodiesterases. In addition, we found that C. elegans phototransduction requires LITE-1, a candidate photoreceptor protein known to be a member of the invertebrate taste receptor family. Our genetic, pharmacological and electrophysiological data suggest a model in which LITE-1 transduces light signals in ASJ via G protein signaling, which leads to upregulation of the second messenger cGMP, followed by opening of cGMP-sensitive CNG channels and stimulation of photoreceptor cells. Our results identify a phototransduction cascade in C. elegans and implicate the function of a 'taste receptor' in phototransduction. |
| 巻・号 | 13(6) |
| ページ | 715-22 |
| 公開日 | 2010-6-1 |
| DOI | 10.1038/nn.2540 |
| PII | nn.2540 |
| PMID | 20436480 |
| PMC | PMC2882063 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cyclic GMP / metabolism* Cyclic Nucleotide-Gated Cation Channels / metabolism GTP-Binding Proteins / metabolism* Guanylate Cyclase / metabolism Light Signal Transduction / physiology* Membrane Potentials / physiology Membrane Proteins / genetics Membrane Proteins / metabolism* Models, Neurological Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurons / physiology Phosphoric Diester Hydrolases / metabolism Photic Stimulation Photoreceptor Cells, Invertebrate / physiology* Signal Transduction Up-Regulation |
| IF | 20.071 |
| 引用数 | 105 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3098 |