RRC ID 46665
Author Petrella LN, Wang W, Spike CA, Rechtsteiner A, Reinke V, Strome S.
Title synMuv B proteins antagonize germline fate in the intestine and ensure C. elegans survival.
Journal Development
Abstract Previous studies demonstrated that a subset of synMuv B mutants ectopically misexpress germline-specific P-granule proteins in their somatic cells, suggesting a failure to properly orchestrate a soma/germline fate decision. Surprisingly, this fate confusion does not affect viability at low to ambient temperatures. Here, we show that, when grown at high temperature, a majority of synMuv B mutants irreversibly arrest at the L1 stage. High temperature arrest (HTA) is accompanied by upregulation of many genes characteristic of germ line, including genes encoding components of the synaptonemal complex and other meiosis proteins. HTA is suppressed by loss of global regulators of germline chromatin, including MES-4, MRG-1, ISW-1 and the MES-2/3/6 complex, revealing that arrest is caused by somatic cells possessing a germline-like chromatin state. Germline genes are preferentially misregulated in the intestine, and necessity and sufficiency tests demonstrate that the intestine is the tissue responsible for HTA. We propose that synMuv B mutants fail to erase or antagonize an inherited germline chromatin state in somatic cells during embryonic and early larval development. As a consequence, somatic cells gain a germline program of gene expression in addition to their somatic program, leading to a mixed fate. Somatic expression of germline genes is enhanced at elevated temperature, leading to developmentally compromised somatic cells and arrest of newly hatched larvae.
Volume 138(6)
Pages 1069-79
Published 2011-3-1
DOI 10.1242/dev.059501
PII 138/6/1069
PMID 21343362
PMC PMC3042865
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Lineage / genetics* Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Genes, Helminth Germ Cells / growth & development Germ Cells / physiology* Intestinal Mucosa / metabolism Intestines / embryology* Intestines / growth & development Larva / genetics Larva / growth & development Larva / metabolism Larva / physiology Life Cycle Stages / genetics Microarray Analysis Survival / physiology Temperature
IF 5.611
Times Cited 52
C.elegans tm1489