論文 - 詳細
| RRC ID | 46710 |
|---|---|
| 著者 | Ogura K, Okada T, Mitani S, Gengyo-Ando K, Baillie DL, Kohara Y, Goshima Y. |
| タイトル | Protein phosphatase 2A cooperates with the autophagy-related kinase UNC-51 to regulate axon guidance in Caenorhabditis elegans. |
| ジャーナル | Development |
| Abstract |
UNC-51 is a serine/threonine protein kinase conserved from yeast to humans. The yeast homolog Atg1 regulates autophagy (catabolic membrane trafficking) required for surviving starvation. In C. elegans, UNC-51 regulates the axon guidance of many neurons by a different mechanism than it and its homologs use for autophagy. UNC-51 regulates the subcellular localization (trafficking) of UNC-5, a receptor for the axon guidance molecule UNC-6/Netrin; however, the molecular details of the role for UNC-51 are largely unknown. Here, we report that UNC-51 physically interacts with LET-92, the catalytic subunit of serine/threonine protein phosphatase 2A (PP2A-C), which plays important roles in many cellular functions. A low allelic dose of LET-92 partially suppressed axon guidance defects of weak, but not severe, unc-51 mutants, and a low allelic dose of PP2A regulatory subunits A (PAA-1/PP2A-A) and B (SUR-6/PP2A-B) partially enhanced the weak unc-51 mutants. We also found that LET-92 can work cell-non-autonomously on axon guidance in neurons, and that LET-92 colocalized with UNC-51 in neurons. In addition, PP2A dephosphorylated phosphoproteins that had been phosphorylated by UNC-51. These results suggest that, by forming a complex, PP2A cooperates with UNC-51 to regulate axon guidance by regulating phosphorylation. This is the first report of a serine/threonine protein phosphatase functioning in axon guidance in vivo. |
| 巻・号 | 137(10) |
| ページ | 1657-67 |
| 公開日 | 2010-5-1 |
| DOI | 10.1242/dev.050708 |
| PII | dev.050708 |
| PMID | 20392746 |
| PMC | PMC3188576 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Autophagy / genetics Autophagy / physiology Axonal Transport / genetics Axonal Transport / physiology Axons / metabolism Axons / physiology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Molecular Sequence Data Neurogenesis / genetics Neurogenesis / physiology Phosphorylation / genetics Protein Binding / physiology Protein Kinases / metabolism Protein Kinases / physiology Protein Phosphatase 2 / genetics Protein Phosphatase 2 / metabolism Protein Phosphatase 2 / physiology* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Protein Serine-Threonine Kinases / physiology* Protein Subunits / genetics Protein Subunits / metabolism Sequence Homology, Amino Acid Synaptic Transmission / genetics Synaptic Transmission / physiology |
| IF | 5.611 |
| 引用数 | 27 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 線虫 | tm655 |