Reference - Detail
| RRC ID | 46998 |
|---|---|
| Author | Dong Q, Yin FX, Gao F, Shen Y, Zhang F, Li Y, He H, Gonzalez M, Yang J, Zhang S, Su M, Chen YH, Li F. |
| Title | Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading. |
| Journal | Mol Cell |
| Abstract |
CENP-A is a centromere-specific histone 3 variant essential for centromere specification. CENP-A partially replaces canonical histone H3 at the centromeres. How the particular CENP-A/H3 ratio at centromeres is precisely maintained is unknown. It also remains unclear how CENP-A is excluded from non-centromeric chromatin. Here, we identify Ccp1, an uncharacterized NAP family protein in fission yeast that antagonizes CENP-A loading at both centromeric and non-centromeric regions. Like the CENP-A loading factor HJURP, Ccp1 interacts with CENP-A and is recruited to centromeres at the end of mitosis in a Mis16-dependent manner. These data indicate that factors with opposing CENP-A loading activities are recruited to centromeres. Furthermore, Ccp1 also cooperates with H2A.Z to evict CENP-A assembled in euchromatin. Structural analyses indicate that Ccp1 forms a homodimer that is required for its anti-CENP-A loading activity. Our study establishes mechanisms for maintenance of CENP-A homeostasis at centromeres and the prevention of ectopic assembly of centromeres. |
| Volume | 64(1) |
| Pages | 79-91 |
| Published | 2016-10-6 |
| DOI | 10.1016/j.molcel.2016.08.022 |
| PMID | 27666591 |
| PMC | PMC5055466 |
| MeSH | Binding Sites Carboxypeptidases / chemistry Carboxypeptidases / genetics* Carboxypeptidases / metabolism Carrier Proteins / chemistry Carrier Proteins / genetics* Carrier Proteins / metabolism Centromere / chemistry Centromere / metabolism Centromere / ultrastructure Chromatin Assembly and Disassembly Chromosomal Proteins, Non-Histone / chemistry Chromosomal Proteins, Non-Histone / genetics* Chromosomal Proteins, Non-Histone / metabolism Euchromatin / chemistry* Euchromatin / metabolism Euchromatin / ultrastructure Gene Expression Regulation, Fungal* Histones / chemistry Histones / genetics Histones / metabolism Mitosis Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Protein Multimerization Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Signal Transduction |
| IF | 15.584 |
| Times Cited | 8 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Yeast | S. pombe strains |