Reference - Detail
| RRC ID | 47223 |
|---|---|
| Author | Foglizzo M, Middleton AJ, Day CL. |
| Title | Structure and Function of the RING Domains of RNF20 and RNF40, Dimeric E3 Ligases that Monoubiquitylate Histone H2B. |
| Journal | J Mol Biol |
| Abstract |
Monoubiquitylation of histone H2B is a post-translational mark that plays key roles in regulation of transcription and genome stability. In humans, attachment of ubiquitin to lysine 120 of histone H2B depends on the activity of the E2 ubiquitin-conjugating enzyme, Ube2B, and the really interesting new gene (RING) E3 ligases, RING finger protein (RNF) 20 and RNF40. To better understand the molecular basis of this modification, we have solved the crystal structure of the RNF20 RING domain and show that it is a homodimer that specifically interacts with the Ube2B~Ub conjugate. By mutating residues at the E3-E2 and E3-ubiquitin interfaces, we identify key contacts required for interaction of the RNF20 RING domain with the Ube2B~Ub conjugate. These mutants were used to generate a structure-based model of the RNF20-Ube2B~Ub complex that reveals differences from other RING-E2~Ub complexes, and suggests how the RNF20-Ube2B~Ub complex might interact with its nucleosomal substrate. Additionally, we show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active. Together, our studies provide new insights into the mechanisms that regulate RNF20-mediated ubiquitin transfer from Ube2B. |
| Volume | 428(20) |
| Pages | 4073-4086 |
| Published | 2016-10-9 |
| DOI | 10.1016/j.jmb.2016.07.025 |
| PII | S0022-2836(16)30339-4 |
| PMID | 27569044 |
| MeSH | Crystallography, X-Ray DNA Mutational Analysis Histones / metabolism* Models, Molecular Protein Binding Protein Conformation Protein Domains Protein Multimerization Ubiquitin / metabolism* Ubiquitin-Protein Ligases / chemistry* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* |
| IF | 4.76 |
| Times Cited | 5 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Genome Network Project Human cDNA IRAK004G03 (HGX001747). |