論文 - 詳細
| RRC ID | 47466 |
|---|---|
| 著者 | Zhang JM, Liu XM, Ding YH, Xiong LY, Ren JY, Zhou ZX, Wang HT, Zhang MJ, Yu Y, Dong MQ, Du LL. |
| タイトル | Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2. |
| ジャーナル | PLoS Biol |
| Abstract |
Structure-specific nucleases play crucial roles in many DNA repair pathways. They must be precisely controlled to ensure optimal repair outcomes; however, mechanisms of their regulation are not fully understood. Here, we report a fission yeast protein, Pxd1, that binds to and regulates two structure-specific nucleases: Rad16XPF-Swi10ERCC1 and Dna2-Cdc24. Strikingly, Pxd1 influences the activities of these two nucleases in opposite ways: It activates the 3' endonuclease activity of Rad16-Swi10 but inhibits the RPA-mediated activation of the 5' endonuclease activity of Dna2. Pxd1 is required for Rad16-Swi10 to function in single-strand annealing, mating-type switching, and the removal of Top1-DNA adducts. Meanwhile, Pxd1 attenuates DNA end resection mediated by the Rqh1-Dna2 pathway. Disabling the Dna2-inhibitory activity of Pxd1 results in enhanced use of a break-distal repeat sequence in single-strand annealing and a greater loss of genetic information. We propose that Pxd1 promotes proper DNA repair by differentially regulating two structure-specific nucleases. |
| 巻・号 | 12(9) |
| ページ | e1001946 |
| 公開日 | 2014-9-1 |
| DOI | 10.1371/journal.pbio.1001946 |
| PII | PBIOLOGY-D-14-01485 |
| PMID | 25203555 |
| PMC | PMC4159138 |
| MeSH | Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism DNA Damage DNA Helicases / genetics DNA Helicases / metabolism DNA Repair* DNA Topoisomerases, Type I / genetics DNA Topoisomerases, Type I / metabolism DNA, Fungal / genetics* DNA, Fungal / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Flap Endonucleases / antagonists & inhibitors Flap Endonucleases / genetics* Flap Endonucleases / metabolism Gene Expression Regulation, Fungal* Protein Binding Schizosaccharomyces / enzymology Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / agonists Schizosaccharomyces pombe Proteins / antagonists & inhibitors Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Signal Transduction |
| IF | 7.076 |
| 引用数 | 9 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | S. pombe ORFeome clone SpYFH33E03 (SPW093299) SpYFH34B04 (SPW093628) SpYFH47F11 (SPW098931). |