| RRC ID |
47466
|
| 著者 |
Zhang JM, Liu XM, Ding YH, Xiong LY, Ren JY, Zhou ZX, Wang HT, Zhang MJ, Yu Y, Dong MQ, Du LL.
|
| タイトル |
Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.
|
| ジャーナル |
PLoS Biol
|
| Abstract |
Structure-specific nucleases play crucial roles in many DNA repair pathways. They must be precisely controlled to ensure optimal repair outcomes; however, mechanisms of their regulation are not fully understood. Here, we report a fission yeast protein, Pxd1, that binds to and regulates two structure-specific nucleases: Rad16XPF-Swi10ERCC1 and Dna2-Cdc24. Strikingly, Pxd1 influences the activities of these two nucleases in opposite ways: It activates the 3' endonuclease activity of Rad16-Swi10 but inhibits the RPA-mediated activation of the 5' endonuclease activity of Dna2. Pxd1 is required for Rad16-Swi10 to function in single-strand annealing, mating-type switching, and the removal of Top1-DNA adducts. Meanwhile, Pxd1 attenuates DNA end resection mediated by the Rqh1-Dna2 pathway. Disabling the Dna2-inhibitory activity of Pxd1 results in enhanced use of a break-distal repeat sequence in single-strand annealing and a greater loss of genetic information. We propose that Pxd1 promotes proper DNA repair by differentially regulating two structure-specific nucleases.
|
| 巻・号 |
12(9)
|
| ページ |
e1001946
|
| 公開日 |
2014-9-1
|
| DOI |
10.1371/journal.pbio.1001946
|
| PII |
PBIOLOGY-D-14-01485
|
| PMID |
25203555
|
| PMC |
PMC4159138
|
| MeSH |
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
DNA Damage
DNA Helicases / genetics
DNA Helicases / metabolism
DNA Repair*
DNA Topoisomerases, Type I / genetics
DNA Topoisomerases, Type I / metabolism
DNA, Fungal / genetics*
DNA, Fungal / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Flap Endonucleases / antagonists & inhibitors
Flap Endonucleases / genetics*
Flap Endonucleases / metabolism
Gene Expression Regulation, Fungal*
Protein Binding
Schizosaccharomyces / enzymology
Schizosaccharomyces / genetics*
Schizosaccharomyces pombe Proteins / agonists
Schizosaccharomyces pombe Proteins / antagonists & inhibitors
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
Signal Transduction
|
| IF |
7.076
|
| 引用数 |
9
|
|
WOS 分野
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
S. pombe ORFeome clone SpYFH33E03 (SPW093299)
SpYFH34B04 (SPW093628)
SpYFH47F11 (SPW098931). |
