Reference - Detail
| RRC ID | 47483 |
|---|---|
| Author | Boehringer J, Riedinger C, Paraskevopoulos K, Johnson EO, Lowe ED, Khoudian C, Smith D, Noble ME, Gordon C, Endicott JA. |
| Title | Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. |
| Journal | Biochem J |
| Abstract |
The ubiquitin-proteasome system targets selected proteins for degradation by the 26S proteasome. Rpn12 is an essential component of the 19S regulatory particle and plays a role in recruiting the extrinsic ubiquitin receptor Rpn10. In the present paper we report the crystal structure of Rpn12, a proteasomal PCI-domain-containing protein. The structure helps to define a core structural motif for the PCI domain and identifies potential sites through which Rpn12 might form protein-protein interactions. We demonstrate that mutating residues at one of these sites impairs Rpn12 binding to Rpn10 in vitro and reduces Rpn10 incorporation into proteasomes in vivo. |
| Volume | 448(1) |
| Pages | 55-65 |
| Published | 2012-11-15 |
| DOI | 10.1042/BJ20120542 |
| PII | BJ20120542 |
| PMID | 22906049 |
| PMC | PMC3481250 |
| MeSH | Animals Arabidopsis Proteins / chemistry COP9 Signalosome Complex Carrier Proteins / chemistry Carrier Proteins / metabolism* Circular Dichroism Crystallography, X-Ray Drosophila Proteins / chemistry Microtubule-Associated Proteins / chemistry Models, Molecular Mutagenesis, Site-Directed Peptide Fragments / chemistry Peptide Fragments / metabolism Proteasome Endopeptidase Complex / metabolism* Protein Binding Protein Conformation Protein Interaction Mapping Protein Structure, Tertiary RNA-Binding Proteins Recombinant Proteins / metabolism Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / chemistry* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Structure-Activity Relationship Ubiquitin / metabolism Winged-Helix Transcription Factors / chemistry |
| IF | 4.097 |
| Times Cited | 14 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| DNA material | pDUAL (RDB06178) |