RRC ID 47751
Author Suzuki KT, Suzuki M, Shigeta M, Fortriede JD, Takahashi S, Mawaribuchi S, Yamamoto T, Taira M, Fukui A.
Title Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis.
Journal Dev Biol
Abstract Keratin genes belong to the intermediate filament superfamily and their expression is altered following morphological and physiological changes in vertebrate epithelial cells. Keratin genes are divided into two groups, type I and II, and are clustered on vertebrate genomes, including those of Xenopus species. Various keratin genes have been identified and characterized by their unique expression patterns throughout ontogeny in Xenopus laevis; however, compilation of previously reported and newly identified keratin genes in two Xenopus species is required for our further understanding of keratin gene evolution, not only in amphibians but also in all terrestrial vertebrates. In this study, 120 putative type I and II keratin genes in total were identified based on the genome data from two Xenopus species. We revealed that most of these genes are highly clustered on two homeologous chromosomes, XLA9_10 and XLA2 in X. laevis, and XTR10 and XTR2 in X. tropicalis, which are orthologous to those of human, showing conserved synteny among tetrapods. RNA-Seq data from various embryonic stages and adult tissues highlighted the unique expression profiles of orthologous and homeologous keratin genes in developmental stage- and tissue-specific manners. Moreover, we identified dozens of epidermal keratin proteins from the whole embryo, larval skin, tail, and adult skin using shotgun proteomics. In light of our results, we discuss the radiation, diversification, and unique expression of the clustered keratin genes, which are closely related to epidermal development and terrestrial adaptation during amphibian evolution, including Xenopus speciation.
Volume 426(2)
Pages 384-392
Published 2017-6-15
DOI 10.1016/j.ydbio.2016.10.018
PII S0012-1606(16)30091-4
PMID 27842699
MeSH Animals Diploidy Epidermis / growth & development Epidermis / metabolism Evolution, Molecular Gene Expression Profiling Gene Expression Regulation, Developmental* Genome Genomics Keratins / genetics* Multigene Family / genetics* Phylogeny Proteomics / methods Species Specificity Spectrometry, Mass, Electrospray Ionization Tetraploidy Transcriptome Xenopus / genetics* Xenopus / metabolism Xenopus Proteins / genetics* Xenopus laevis / genetics Xenopus laevis / metabolism
IF 2.896
Times Cited 7
Clawed frogs / Newts X. tropicalis