RRC ID 4792
Author Suzuki K, Takahashi S, Haramoto Y, Onuma Y, Nagamine K, Okabayashi K, Hashizume K, Iwanaka T, Asashima M.
Title XRASGRP2 is essential for blood vessel formation during Xenopus development.
Journal Int. J. Dev. Biol.
Abstract Ras guanyl nucleotide-releasing protein 2 (RASGRP2), one of the Ras guanine exchange factors, is implicated as a critical regulator of inside-out integrin activation in human lymphocytes, neutrophils and platelets. However, the activities of this protein in endothelial cells remain unclear. In the current study, we identify a physiological function in blood vessel formation for XRASGRP2, which is the Xenopus ortholog of mammalian RASGRP2. XRASGRP2 over-expression induced ectopic vascular formation, and XRASGRP2-knockdown embryos showed delayed vascular development. We also investigated the upstream signaling of XRASGRP2 in endothelium formation. XRASGRP2 expression was up-regulated in the presence of VEGF-A and down-regulated following VEGF-A depletion. XRASGRP2 knockdown abolished the ectopic induction of endothelial cells by VEGF-A in the posterior ventral blood island. These results suggest that XRASGRP2 is essential for vascular formation during Xenopus development.
Volume 54(4)
Pages 609-15
Published 2010
DOI 10.1387/ijdb.092929ks
PII 092929ks
PMID 19598105
MeSH Animals Blood Vessels / metabolism* Cell Differentiation / genetics Endothelial Cells / metabolism Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism Humans Signal Transduction / physiology Vascular Endothelial Growth Factor A / genetics Vascular Endothelial Growth Factor A / metabolism Xenopus / genetics Xenopus / metabolism* Xenopus Proteins / genetics Xenopus Proteins / physiology* ras Guanine Nucleotide Exchange Factors / genetics ras Guanine Nucleotide Exchange Factors / physiology*
IF 1.092
Times Cited 4
Xenopus tropicalis