| RRC ID |
4820
|
| Author |
Komatsu K, Nishikawa Y, Ohtsuka T, Taji T, Quatrano RS, Tanaka S, Sakata Y.
|
| Title |
Functional analyses of the ABI1-related protein phosphatase type 2C reveal evolutionarily conserved regulation of abscisic acid signaling between Arabidopsis and the moss Physcomitrella patens.
|
| Journal |
Plant Mol Biol
|
| Abstract |
We employed a comparative genomic approach to understand protein phosphatase 2C (PP2C)-mediated abscisic acid (ABA) signaling in the moss Physcomitrella patens. Ectopic expression of Arabidopsis (Arabidopsis thaliana) abi1-1, a dominant mutant allele of ABI1 encoding a PP2C involved in the negative regulation of ABA signaling, caused ABA insensitivity of P. patens both in gene expression of late embryogenesis abundant (LEA) genes and in ABA-induced protonemal growth inhibition. The transgenic abi1-1 plants showed decreased ABA-induced freezing tolerance, and decreased tolerance to osmotic stress. Analyses of the P. patens genome revealed that only two (PpABI1A and PpABI1B) PP2C genes were related to ABI1. In the ppabi1a null mutants, ABA-induced expression of LEA genes was elevated, and protonemal growth was inhibited with lower ABA concentration compared to the wild type. Moreover, ABA-induced freezing tolerance of the ppabi1a mutants was markedly enhanced. We provide the genetic evidence that PP2C-mediated ABA signaling is evolutionarily conserved between Arabidopsis and P. patens.
|
| Volume |
70(3)
|
| Pages |
327-40
|
| Published |
2009-6-1
|
| DOI |
10.1007/s11103-009-9476-z
|
| PMID |
19266168
|
| MeSH |
Abscisic Acid / metabolism*
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
Bryopsida / enzymology*
Bryopsida / genetics
Cloning, Molecular
Evolution, Molecular
Gene Expression Regulation, Plant
Genome, Plant
Molecular Sequence Data
Mutation
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
Phylogeny
Plants, Genetically Modified / enzymology
Plants, Genetically Modified / genetics
Protein Phosphatase 2C
RNA, Plant / genetics
Signal Transduction*
Stress, Physiological
|
| IF |
3.302
|
| Times Cited |
66
|
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pdp31463 |
