論文 - 詳細
| RRC ID | 48794 |
|---|---|
| 著者 | Ataeian M, Tegha-Dunghu J, Curtis DG, Sykes EM, Nozohourmehrabad A, Bajaj M, Cheung K, Srayko M. |
| タイトル | Maternal MEMI Promotes Female Meiosis II in Response to Fertilization in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
In most animals, female meiosis completes only after fertilization. Sperm entry has been implicated in providing a signal for the initiation of the final meiotic processes; however, a maternal component required for this process has not been previously identified. We report the characterization of a novel family of three highly similar paralogs (memi-1, memi-2, memi-3) that encode oocyte-specific proteins. A hyper-morphic mutation memi-1(sb41) results in failure to exit female meiosis II properly; however, loss of all three paralogs results in a "skipped meiosis II" phenotype. Mutations that prevent fertilization, such as fer-1(hc1), also cause a skipped meiosis II phenotype, suggesting that the MEMI proteins represent a maternal component of a postfertilization signal that specifies the meiosis II program. MEMI proteins are degraded before mitosis and sensitive to ZYG-11, a substrate-specific adapter for cullin-based ubiquitin ligase activity, and the memi-1(sb41) mutation results in inappropriate persistence of the MEMI-1 protein into mitosis. Using an RNAi screen for suppressors of memi-1(sb41), we identified a sperm-specific PP1 phosphatase, GSP-3/4, as a putative sperm component of the MEMI pathway. We also found that MEMI and GSP-3/4 proteins can physically interact via co-immunoprecipitation. These results suggest that sperm-specific PP1 and maternal MEMI proteins act in the same pathway after fertilization to facilitate proper meiosis II and the transition into embryonic mitosis. |
| 巻・号 | 204(4) |
| ページ | 1461-1477 |
| 公開日 | 2016-12-1 |
| DOI | 10.1534/genetics.116.192997 |
| PII | genetics.116.192997 |
| PMID | 27729423 |
| PMC | PMC5161279 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Female Fertilization / genetics* Male Meiosis / genetics* Membrane Proteins / genetics Membrane Proteins / metabolism Mutation Oocytes / cytology Oocytes / metabolism* Protein Binding Proteolysis |
| IF | 4.015 |
| 引用数 | 2 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3099 tm2638 tm3158 |