RRC ID |
49338
|
著者 |
Zafar F, Okita AK, Onaka AT, Su J, Katahira Y, Nakayama JI, Takahashi TS, Masukata H, Nakagawa T.
|
タイトル |
Regulation of mitotic recombination between DNA repeats in centromeres.
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ジャーナル |
Nucleic Acids Res
|
Abstract |
Centromeres that are essential for faithful segregation of chromosomes consist of unique DNA repeats in many eukaryotes. Although recombination is under-represented around centromeres during meiosis, little is known about recombination between centromere repeats in mitotic cells. Here, we compared spontaneous recombination that occurs between ade6B/ade6X inverted repeats integrated at centromere 1 (cen1) or at a non-centromeric ura4 locus in fission yeast. Remarkably, distinct mechanisms of homologous recombination (HR) were observed in centromere and non-centromere regions. Rad51-dependent HR that requires Rad51, Rad54 and Rad52 was predominant in the centromere, whereas Rad51-independent HR that requires Rad52 also occurred in the arm region. Crossovers between inverted repeats (i.e. inversions) were under-represented in the centromere as compared to the arm region. While heterochromatin was dispensable, Mhf1/CENP-S, Mhf2/CENP-X histone-fold proteins and Fml1/FANCM helicase were required to suppress crossovers. Furthermore, Mhf1 and Fml1 were found to prevent gross chromosomal rearrangements mediated by centromere repeats. These data for the first time uncovered the regulation of mitotic recombination between DNA repeats in centromeres and its physiological role in maintaining genome integrity.
|
巻・号 |
45(19)
|
ページ |
11222-11235
|
公開日 |
2017-11-2
|
DOI |
10.1093/nar/gkx763
|
PII |
4093170
|
PMID |
28977643
|
PMC |
PMC5737691
|
MeSH |
Centromere / genetics*
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
DNA Helicases / genetics
DNA Helicases / metabolism
DNA, Fungal / genetics*
DNA, Fungal / metabolism
Genome, Fungal / genetics
Homologous Recombination*
Mitosis / genetics*
Models, Genetic
Rad51 Recombinase / genetics
Rad51 Recombinase / metabolism
Rad52 DNA Repair and Recombination Protein / genetics
Rad52 DNA Repair and Recombination Protein / metabolism
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
|
IF |
11.502
|
引用数 |
9
|
リソース情報 |
酵母 |
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