論文 - 詳細
| RRC ID | 49345 |
|---|---|
| 著者 | Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A. |
| タイトル | An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells. |
| ジャーナル | Stem Cell Reports |
| Abstract |
During liver development, hepatoblasts and liver non-parenchymal cells (NPCs) such as liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs) constitute the liver bud where they proliferate and differentiate. Accordingly, we reasoned that liver NPCs would support the maturation of hepatocytes derived from human induced pluripotent stem cells (hiPSCs), which usually exhibit limited functions. We found that the transforming growth factor β and Rho signaling pathways, respectively, regulated the proliferation and maturation of LSEC and HSC progenitors isolated from mouse fetal livers. Based on these results, we have established culture systems to generate LSECs and HSCs from hiPSCs. These hiPSC-derived NPCs exhibited distinctive phenotypes and promoted self-renewal of hiPSC-derived liver progenitor cells (LPCs) over the long term in the two-dimensional culture system without exogenous cytokines and hepatic maturation of hiPSC-derived LPCs. Thus, a functional human liver model can be constructed in vitro from the LPCs, LSECs, and HSCs derived from hiPSCs. |
| 巻・号 | 9(2) |
| ページ | 490-498 |
| 公開日 | 2017-8-8 |
| DOI | 10.1016/j.stemcr.2017.06.010 |
| PII | S2213-6711(17)30273-4 |
| PMID | 28757162 |
| PMC | PMC5549957 |
| MeSH | Activated-Leukocyte Cell Adhesion Molecule / metabolism Animals Biomarkers Cell Differentiation* / genetics Cells, Cultured Coculture Techniques Endothelial Cells / cytology Endothelial Cells / metabolism Fetus Gene Expression Profiling Hepatic Stellate Cells / cytology Hepatic Stellate Cells / metabolism Hepatocytes / cytology Hepatocytes / metabolism Humans Immunophenotyping Induced Pluripotent Stem Cells / cytology* Liver / cytology* Liver / metabolism Liver Regeneration Mice Phenotype |
| IF | 6.032 |
| 引用数 | 36 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | 409B2(HPS0076) 454E2(HPS0077) |