RRC ID 49510
Author Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HW.
Title ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.
Journal Mol Biol Cell
Abstract Small GTPases of the ADP-ribosylation factor (ARF) family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. We recently showed that class I ARFs (ARF1 and ARF3) localize to recycling endosomes, as well as to the Golgi, and are redundantly required for recycling of endocytosed transferrin. On the other hand, the roles of class II ARFs (ARF4 and ARF5) are not yet fully understood, and the complementary or overlapping functions of class I and class II ARFs have been poorly characterized. In this study, we find that simultaneous depletion of ARF1 and ARF4 induces extensive tubulation of recycling endosomes. Moreover, the depletion of ARF1 and ARF4 inhibits retrograde transport of TGN38 and mannose-6-phosphate receptor from early/recycling endosomes to the trans-Golgi network (TGN) but does not affect the endocytic/recycling pathway of transferrin receptor or inhibit retrograde transport of CD4-furin from late endosomes to the TGN. These observations indicate that the ARF1+ARF4 and ARF1+ARF3 pairs are both required for integrity of recycling endosomes but are involved in distinct transport pathways: the former pair regulates retrograde transport from endosomes to the TGN, whereas the latter is required for the transferrin recycling pathway from endosomes to the plasma membrane.
Volume 24(16)
Pages 2570-81
Published 2013-8-1
DOI 10.1091/mbc.E13-04-0197
PII mbc.E13-04-0197
PMID 23783033
PMC PMC3744953
MeSH ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism* CD4 Antigens / metabolism Cell Line, Tumor Endosomes / metabolism* Furin / metabolism Golgi Apparatus / metabolism* HeLa Cells Humans Membrane Glycoproteins / metabolism Protein Transport RNA Interference RNA, Small Interfering Receptor, IGF Type 2 / metabolism Receptors, Transferrin / metabolism rab GTP-Binding Proteins / metabolism rab4 GTP-Binding Proteins / metabolism trans-Golgi Network / metabolism
IF 3.791
Times Cited 29
DNA material pCAG/hArf4(WT)-HA (RDB15354) pCAG/hArf4(Q71L)-HA (RDB15355) pCAG/hArf4(T31N)-HA (RDB15356) pCAG/hArf4(WT)-mCherry (RDB15357) pCAG/hArf4(WT)-EGFP (RDB15358).