論文 - 詳細
| RRC ID | 49560 |
|---|---|
| 著者 | Shamim HM, Minami Y, Tanaka D, Ukimori S, Murray JM, Ueno M. |
| タイトル | Fission yeast strains with circular chromosomes require the 9-1-1 checkpoint complex for the viability in response to the anti-cancer drug 5-fluorodeoxyuridine. |
| ジャーナル | PLoS One |
| Abstract |
Thymidine kinase converts 5-fluorodeoxyuridine to 5-fluorodeoxyuridine monophosphate, which causes disruption of deoxynucleotide triphosphate ratios. The fission yeast Schizosaccharomyces pombe does not express endogenous thymidine kinase but 5-fluorodeoxyuridine inhibits growth when exogenous thymidine kinase is expressed. Unexpectedly, we found that 5-fluorodeoxyuridine causes S phase arrest even without thymidine kinase expression. DNA damage checkpoint proteins such as the 9-1-1 complex were required for viability in the presence of 5-fluorodeoxyuridine. We also found that strains with circular chromosomes, due to loss of pot1+, which have higher levels of replication stress, were more sensitive to loss of the 9-1-1 complex in the presence of 5-fluorodeoxyuridine. Thus, our results suggest that strains carrying circular chromosomes exhibit a greater dependence on DNA damage checkpoints to ensure viability in the presence of 5-fluorodeoxyuridine compared to stains that have linear chromosomes. |
| 巻・号 | 12(11) |
| ページ | e0187775 |
| 公開日 | 2017-11-9 |
| DOI | 10.1371/journal.pone.0187775 |
| PII | PONE-D-17-16192 |
| PMID | 29121084 |
| PMC | PMC5679574 |
| MeSH | Antineoplastic Agents / pharmacology* Chromosome Segregation / drug effects Chromosomes, Fungal / genetics* DNA Damage DNA Replication / drug effects DNA, Fungal / genetics* Floxuridine / pharmacology* Microbial Viability / drug effects* Mutation Mycoses S Phase Cell Cycle Checkpoints / drug effects S Phase Cell Cycle Checkpoints / genetics Schizosaccharomyces / cytology Schizosaccharomyces / drug effects Schizosaccharomyces / genetics* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Species Specificity Telomere / genetics |
| IF | 2.74 |
| 引用数 | 0 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY18372 FY18394 |