| RRC ID |
50628
|
| 著者 |
Zhou Q, Zhou YN, Jin DJ, Tse-Dinh YC.
|
| タイトル |
Deacetylation of topoisomerase I is an important physiological function of E. coli CobB.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Escherichia coli topoisomerase I (TopA), a regulator of global and local DNA supercoiling, is modified by Nε-Lysine acetylation. The NAD+-dependent protein deacetylase CobB can reverse both enzymatic and non-enzymatic lysine acetylation modification in E. coli. Here, we show that the absence of CobB in a ΔcobB mutant reduces intracellular TopA catalytic activity and increases negative DNA supercoiling. TopA expression level is elevated as topA transcription responds to the increased negative supercoiling. The slow growth phenotype of the ΔcobB mutant can be partially compensated by further increase of intracellular TopA level via overexpression of recombinant TopA. The relaxation activity of purified TopA is decreased by in vitro non-enzymatic acetyl phosphate mediated lysine acetylation, and the presence of purified CobB protects TopA from inactivation by such non-enzymatic acetylation. The specific activity of TopA expressed from His-tagged fusion construct in the chromosome is inversely proportional to the degree of in vivo lysine acetylation during growth transition and growth arrest. These findings demonstrate that E. coli TopA catalytic activity can be modulated by lysine acetylation-deacetylation, and prevention of TopA inactivation from excess lysine acetylation and consequent increase in negative DNA supercoiling is an important physiological function of the CobB protein deacetylase.
|
| 巻・号 |
45(9)
|
| ページ |
5349-5358
|
| 公開日 |
2017-5-19
|
| DOI |
10.1093/nar/gkx250
|
| PII |
3294615
|
| PMID |
28398568
|
| PMC |
PMC5605244
|
| MeSH |
Acetylation
Biocatalysis
Chromosomes, Bacterial / metabolism
DNA Topoisomerases, Type I / metabolism*
DNA, Bacterial / metabolism
DNA, Superhelical / metabolism
Electrophoresis, Agar Gel
Escherichia coli / growth & development
Escherichia coli / metabolism*
Escherichia coli Proteins / metabolism*
Lysine / metabolism
Mutation / genetics
Phenotype
Protein Binding
Proteomics
Recombinant Proteins / metabolism
Sirtuins / metabolism*
Solubility
|
| IF |
11.502
|
| 引用数 |
12
|
| リソース情報 |
| 原核生物(大腸菌) |
ASKA plasmid pCA24N |
