RRC ID 51288
著者 Linsley JW, Hsu IU, Wang W, Kuwada JY.
タイトル Transport of the alpha subunit of the voltage gated L-type calcium channel through the sarcoplasmic reticulum occurs prior to localization to triads and requires the beta subunit but not Stac3 in skeletal muscles.
ジャーナル Traffic
Abstract Contraction of skeletal muscle is initiated by excitation-contraction (EC) coupling during which membrane voltage is transduced to intracellular Ca2+ release. EC coupling requires L-type voltage gated Ca2+ channels (the dihydropyridine receptor or DHPR) located at triads, which are junctions between the transverse (T) tubule and sarcoplasmic reticulum (SR) membranes, that sense membrane depolarization in the T tubule membrane. Reduced EC coupling is associated with ageing, and disruptions of EC coupling result in congenital myopathies for which there are few therapies. The precise localization of DHPRs to triads is critical for EC coupling, yet trafficking of the DHPR to triads is not well understood. Using dynamic imaging of zebrafish muscle fibers, we find that DHPR is transported along the longitudinal SR in a microtubule-independent mechanism. Furthermore, transport of DHPR in the SR membrane is differentially affected in null mutants of Stac3 or DHPRβ, two essential components of EC coupling. These findings reveal previously unappreciated features of DHPR motility within the SR prior to assembly at triads.
巻・号 18(9)
ページ 622-632
公開日 2017-9-1
DOI 10.1111/tra.12502
PMID 28697281
PMC PMC5569907
MeSH Adaptor Proteins, Signal Transducing / metabolism* Animals Calcium / metabolism Calcium Channels, L-Type / metabolism* Cells, Cultured Excitation Contraction Coupling / physiology Muscle Fibers, Skeletal / metabolism Muscle Proteins / metabolism Muscle, Skeletal / metabolism* Sarcoplasmic Reticulum / metabolism* Zebrafish Zebrafish Proteins / metabolism*
IF 4.045
引用数 6
リソース情報
ゼブラフィッシュ cacnb1