Reference - Detail
|Author||Ryu JS, Koo HS.|
|Title||The Caenorhabditis elegans WRN helicase promotes double-strand DNA break repair by mediating end resection and checkpoint activation.|
The protein associated with Werner syndrome (WRN), is involved in DNA repair, checkpoint activation, and telomere maintenance. To better understand the involvement of WRN in double-strand DNA break (DSB) repair, we analyzed the combinatorial role of WRN-1, the Caenorhabditis elegans WRN helicase, in conjunction with EXO-1 and DNA-2 nucleases. We found that WRN-1 cooperates with DNA-2 to resect DSB ends in a pathway acting in parallel to EXO-1. The wrn-1 mutants show an aberrant accumulation of replication protein A (RPA) and RAD-51, and the same pattern of accumulation is also observed in checkpoint-defective strains. We conclude that WRN-1 plays a conserved role in the resection of DSB ends and mediates checkpoint signaling, thereby influencing levels of RPA and RAD-51.
|MeSH||Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Checkpoints* / radiation effects DNA Breaks, Double-Stranded* DNA Helicases / genetics DNA Helicases / metabolism* DNA Repair* / radiation effects Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism Gamma Rays Mutation Rad51 Recombinase / metabolism Replication Protein A / metabolism|