RRC ID 51425
Author Ryu JS, Koo HS.
Title The Caenorhabditis elegans WRN helicase promotes double-strand DNA break repair by mediating end resection and checkpoint activation.
Journal FEBS Lett.
Abstract The protein associated with Werner syndrome (WRN), is involved in DNA repair, checkpoint activation, and telomere maintenance. To better understand the involvement of WRN in double-strand DNA break (DSB) repair, we analyzed the combinatorial role of WRN-1, the Caenorhabditis elegans WRN helicase, in conjunction with EXO-1 and DNA-2 nucleases. We found that WRN-1 cooperates with DNA-2 to resect DSB ends in a pathway acting in parallel to EXO-1. The wrn-1 mutants show an aberrant accumulation of replication protein A (RPA) and RAD-51, and the same pattern of accumulation is also observed in checkpoint-defective strains. We conclude that WRN-1 plays a conserved role in the resection of DSB ends and mediates checkpoint signaling, thereby influencing levels of RPA and RAD-51.
Volume 591(14)
Pages 2155-2166
Published 2017-7
DOI 10.1002/1873-3468.12724
PMID 28640365
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Checkpoints* / radiation effects DNA Breaks, Double-Stranded* DNA Helicases / genetics DNA Helicases / metabolism* DNA Repair* / radiation effects Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism Gamma Rays Mutation Rad51 Recombinase / metabolism Replication Protein A / metabolism
IF 2.675
C.elegans tm1842