論文 - 詳細
| RRC ID | 51515 |
|---|---|
| 著者 | Liu J, Li M, Li L, Chen S, Wang X. |
| タイトル | Ubiquitination of the PI3-kinase VPS-34 promotes VPS-34 stability and phagosome maturation. |
| ジャーナル | J Cell Biol |
| Abstract |
Apoptotic cells generated by programmed cell death are engulfed by phagocytes and enclosed within membrane-bound phagosomes. Maturation of apoptotic cell-containing phagosomes leads to formation of phagolysosomes where cell corpses are degraded. The class III phosphatidylinositol 3-kinase (PI3-kinase) VPS-34 coordinates with PIKI-1, a class II PI3-kinase, to produce PtdIns3P on phagosomes, thus promoting phagosome closure and maturation. Here, we identified UBC-13, an E2 ubiquitin-conjugating enzyme that functions in the same pathway with VPS-34 but in parallel to PIKI-1 to regulate PtdIns3P generation on phagosomes. Loss of ubc-13 affects early steps of phagosome maturation, causing accumulation of cell corpses. We found that UBC-13 functions with UEV-1, a noncatalytic E2 variant, and CHN-1, a U-box-containing E3 ubiquitin ligase, to catalyze K63-linked poly-ubiquitination on VPS-34 both in vitro and in Caenorhabditis elegans Loss of ubc-13, uev-1, or chn-1 disrupts ubiquitin modification of VPS-34 and causes significantly reduced VPS-34 protein levels. Our data suggest that K63-linked ubiquitin modification serves as a general mechanism to modulate VPS-34 stability in multiple processes. |
| 巻・号 | 217(1) |
| ページ | 347-360 |
| 公開日 | 2018-1-2 |
| DOI | 10.1083/jcb.201705116 |
| PII | jcb.201705116 |
| PMID | 29092895 |
| PMC | PMC5748982 |
| MeSH | Animals Apoptosis / physiology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Class III Phosphatidylinositol 3-Kinases / metabolism* Phagocytosis / immunology Phagosomes / metabolism* Phosphatidylinositol 3-Kinases / metabolism Ubiquitin / metabolism Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* Ubiquitination* |
| IF | 8.811 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2692 tm3066 tm3546 |