RRC ID 51536
Author Sato M, Sato K, Tomura K, Kosako H, Sato K.
Title The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans.
Journal Nat Cell Biol
Abstract In Caenorhabditis elegans embryos, paternally provided organelles, including mitochondria, are eliminated by a process of selective autophagy called allophagy, the mechanism by which mitochondrial DNA is inherited maternally. However, it remains unclear how paternal organelles are recognized and targeted for autophagy. Here, we identified an autophagy receptor for allophagy, ALLO-1. ALLO-1 is essential for autophagosome formation around paternal organelles and directly binds to the worm LC3 homologue LGG-1 through its LC3-interacting region (LIR) motif. After fertilization, ALLO-1 accumulates on the paternal organelles before autophagosome formation, and this localization depends on the ubiquitin modification of the paternal organelles. We also identified IKKE-1, a worm homologue of the TBK1 and IKKε family kinase, as another critical regulator of allophagy. IKKE-1 interacts with ALLO-1, and the IKKE-1-dependent phosphorylation of ALLO-1 is important for paternal organelle clearance. Thus, we propose that ALLO-1 is the allophagy receptor whose function is regulated by IKKE-1-dependent phosphorylation.
Volume 20(1)
Pages 81-91
Published 2018-1-1
DOI 10.1038/s41556-017-0008-9
PII 10.1038/s41556-017-0008-9
PMID 29255173
MeSH Animals Autophagosomes Autophagy / genetics* Autophagy-Related Proteins / genetics* Autophagy-Related Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA, Mitochondrial / genetics DNA, Mitochondrial / metabolism Embryo, Nonmammalian Female Gene Expression Regulation Inheritance Patterns* Male Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Mitochondria / metabolism* Phosphorylation Protein-Serine-Threonine Kinases / genetics* Protein-Serine-Threonine Kinases / metabolism Signal Transduction Ubiquitin / genetics Ubiquitin / metabolism Ubiquitination
IF 20.042
Times Cited 12
Resource
C.elegans tm2508 tm4012 tm4102 tm4756