Reference - Detail
| RRC ID | 51829 |
|---|---|
| Author | Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, Yamaji R. |
| Title | The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The majority of studies on possible roles for collagen hydrolysates in human health have focused on their effects on bone and skin. Hydroxyprolyl-glycine (Hyp-Gly) was recently identified as a novel collagen hydrolysate-derived dipeptide in human blood. However, any possible health benefits of Hyp-Gly remain unclear. Here, we report the effects of Hyp-Gly on differentiation and hypertrophy of murine skeletal muscle C2C12 cells. Hyp-Gly increased the fusion index, the myotube size, and the expression of the myotube-specific myosin heavy chain (MyHC) and tropomyosin structural proteins. Hyp-Gly increased the phosphorylation of Akt, mTOR, and p70S6K in myoblasts, whereas the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 inhibited their phosphorylation by Hyp-Gly. LY294002 and the mammalian target of rapamycin (mTOR) inhibitor rapamycin repressed the enhancing effects of Hyp-Gly on MyHC and tropomyosin expression. The peptide/histidine transporter 1 (PHT1) was highly expressed in both myoblasts and myotubes, and co-administration of histidine inhibited Hyp-Gly-induced phosphorylation of p70S6K in myoblasts and myotubes. These results indicate that Hyp-Gly can induce myogenic differentiation and myotube hypertrophy and suggest that Hyp-Gly promotes myogenic differentiation by activating the PI3K/Akt/mTOR signaling pathway, perhaps depending on PHT1 for entry into cells. |
| Volume | 478(3) |
| Pages | 1292-7 |
| Published | 2016-9-23 |
| DOI | 10.1016/j.bbrc.2016.08.114 |
| PII | S0006-291X(16)31378-X |
| PMID | 27553280 |
| MeSH | Animals Cell Differentiation / drug effects* Cell Size / drug effects Collagen / pharmacology* Dipeptides / pharmacology* Histidine / pharmacology Hypertrophy Male Membrane Transport Proteins / metabolism Mice Muscle Fibers, Skeletal / drug effects Muscle Fibers, Skeletal / metabolism Muscle Fibers, Skeletal / pathology* Myoblasts / cytology* Myoblasts / drug effects Myoblasts / metabolism Myosin Heavy Chains / metabolism Phosphatidylinositol 3-Kinases / metabolism Phosphorylation Proto-Oncogene Proteins c-akt / metabolism Ribosomal Protein S6 Kinases, 70-kDa / metabolism Signal Transduction / drug effects TOR Serine-Threonine Kinases / metabolism Tropomyosin / metabolism |
| IF | 2.985 |
| Times Cited | 15 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 14 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |