RRC ID |
52001
|
Author |
Tanaka S, Maekawa A, Matsubara L, Imanishi A, Yano M, Roeder RG, Hasegawa N, Asano S, Ito M.
|
Title |
Periostin supports hematopoietic progenitor cells and niche-dependent myeloblastoma cells in vitro.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
The expression of extracellular matrix protein periostin (POSTN) was attenuated in Med1(-/-) mouse embryonic fibroblasts (MEFs), which exhibited a decreased capability to support hematopoietic progenitor cells (HPCs) in vitro. When bone marrow (BM) cells were cocultured with mitomycin C-treated Med1(+/+) MEFs, or OP-9 or MS-5 BM stromal cells, in the presence of anti-POSTN antibody, the growth of BM cells and number of long-term culture-initiating cells (LTC-ICs) were attenuated. When BM cells were cocultured with Med1(-/-) MEFs in the presence of recombinant POSTN, the growth of BM cells and the number of LTC-ICs were restored. Moreover, antibody-mediated blockage of stromal cells-derived POSTN markedly reduced the growth and cobblestone formation, a leukemic stem cell feature, of stromal cell-dependent MB-1 myeloblastoma cells. POSTN was expressed both in BM cells and variably in different BM stromal cells. Expression in the latter cells was increased by physical interaction with hematopoietic cells. The receptor for POSTN, integrin αvβ3, was expressed abundantly in BM stromal cells. The addition of recombinant POSTN to BM stromal cells induced intracellular signaling downstream of integrin αvβ3. These results suggest that stromal cell POSTN supports both normal HPCs and leukemia-initiating cells in vitro, at least in part, indirectly by acting on stromal cells in an autocrine or paracrine manner.
|
Volume |
478(4)
|
Pages |
1706-12
|
Published |
2016-9-30
|
DOI |
10.1016/j.bbrc.2016.09.008
|
PII |
S0006-291X(16)31457-7
|
PMID |
27596966
|
PMC |
PMC5808943
|
MeSH |
Animals
Blotting, Western
Cell Adhesion Molecules / genetics*
Cell Adhesion Molecules / metabolism
Cell Adhesion Molecules / pharmacology
Cell Line
Cell Line, Tumor
Cell Proliferation / genetics
Cells, Cultured
Coculture Techniques
Embryo, Mammalian / cytology
Fibroblasts / cytology
Fibroblasts / metabolism
Gene Expression*
HEK293 Cells
Hematopoietic Stem Cells / metabolism*
Humans
Integrin alphaVbeta3 / genetics
Integrin alphaVbeta3 / metabolism
Mediator Complex Subunit 1 / genetics
Mediator Complex Subunit 1 / metabolism
Mesenchymal Stem Cells / metabolism
Mice, Inbred C57BL
Mice, Knockout
Reverse Transcriptase Polymerase Chain Reaction
Sarcoma, Myeloid / genetics
Sarcoma, Myeloid / metabolism
Sarcoma, Myeloid / pathology
Signal Transduction / drug effects
Signal Transduction / genetics
Stem Cell Niche*
|
IF |
2.985
|
Times Cited |
4
|
Resource |
Human and Animal Cells |
OP9(RCB1124) |