論文 - 詳細
| RRC ID | 53150 |
|---|---|
| 著者 | Kinoshita A, ten Hove CA, Tabata R, Yamada M, Shimizu N, Ishida T, Yamaguchi K, Shigenobu S, Takebayashi Y, Iuchi S, Kobayashi M, Kurata T, Wada T, Seo M, Hasebe M, Blilou I, Fukuda H, Scheres B, Heidstra R, Kamiya Y, Sawa S. |
| タイトル | A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. |
| ジャーナル | Development |
| Abstract |
The root meristem (RM) is a fundamental structure that is responsible for postembryonic root growth. The RM contains the quiescent center (QC), stem cells and frequently dividing meristematic cells, in which the timing and the frequency of cell division are tightly regulated. In Arabidopsis thaliana, several gain-of-function analyses have demonstrated that peptide ligands of the Clavata3 (CLV3)/embryo surrounding region-related (CLE) family are important for maintaining RM size. Here, we demonstrate that a plant U-box E3 ubiquitin ligase, PUB4, is a novel downstream component of CLV3/CLE signaling in the RM. Mutations in PUB4 reduced the inhibitory effect of exogenous CLV3/CLE peptide on root cell proliferation and columella stem cell maintenance. Moreover, pub4 mutants grown without exogenous CLV3/CLE peptide exhibited characteristic phenotypes in the RM, such as enhanced root growth, increased number of cortex/endodermis stem cells and decreased number of columella layers. Our phenotypic and gene expression analyses indicated that PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/endodermis initial daughters. These data suggest that PUB4 functions as a global regulator of cell proliferation and the timing of asymmetric cell division that are important for final root architecture. |
| 巻・号 | 142(3) |
| ページ | 444-53 |
| 公開日 | 2015-2-1 |
| DOI | 10.1242/dev.113167 |
| PII | 142/3/444 |
| PMID | 25605779 |
| MeSH | Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Asymmetric Cell Division / genetics Asymmetric Cell Division / physiology* Cell Cycle Proteins / metabolism Cell Proliferation / genetics Cell Proliferation / physiology* Cloning, Molecular Cyclins / metabolism Gene Expression Profiling Gene Expression Regulation, Plant / genetics* Meristem / cytology* Microscopy, Confocal Plants, Genetically Modified Signal Transduction / genetics Signal Transduction / physiology* Two-Hybrid System Techniques Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* |
| IF | 5.611 |
| 引用数 | 31 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | psx |