RRC ID 53155
著者 Zach R, Tvaružková J, Schätz M, Tupa O, Grallert B, Prevorovský M.
タイトル Mitotic defects in fission yeast lipid metabolism 'cut' mutants are suppressed by ammonium chloride.
ジャーナル FEMS Yeast Res
Abstract Fission yeast 'cut' mutants show defects in temporal coordination of nuclear division with cytokinesis, resulting in aberrant mitosis and lethality. Among other causes, the 'cut' phenotype can be triggered by genetic or chemical perturbation of lipid metabolism, supposedly resulting in shortage of membrane phospholipids and insufficient nuclear envelope expansion during anaphase. Interestingly, penetrance of the 'cut' phenotype in mutants of the transcription factor cbf11 and acetyl-coenzyme A carboxylase cut6, both related to lipid metabolism, is highly dependent on growth media, although the specific nutrient(s) affecting 'cut' occurrence is not known. In this study, we set out to identify the growth media component(s) responsible for 'cut' phenotype suppression in Δcbf11 and cut6-621 cells. We show that mitotic defects occur rapidly in Δcbf11 cells upon shift from the minimal EMM medium ('cut' suppressing) to the complex YES medium ('cut' promoting). By growing cells in YES medium supplemented with individual EMM components, we identified ammonium chloride, an efficiently utilized nitrogen source, as a specific and potent suppressor of the 'cut' phenotype in both Δcbf11 and cut6-621. Furthermore, we found that ammonium chloride boosts lipid droplet formation in wild-type cells. Our findings suggest a possible involvement of nutrient-responsive signaling in 'cut' suppression.
巻・号 18(6)
公開日 2018-9-1
DOI 10.1093/femsyr/foy064
PII 5040229
PMID 29931271
PMC PMC6037054
MeSH Acetyl-CoA Carboxylase / genetics Ammonium Chloride / chemistry Ammonium Chloride / metabolism Ammonium Chloride / pharmacology* Culture Media / chemistry Lipid Droplets / drug effects Lipid Droplets / metabolism Lipid Metabolism / drug effects* Lipid Metabolism / genetics Mitosis / drug effects* Mitosis / genetics Mutation Penetrance Phenotype Schizosaccharomyces / drug effects* Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Transcription Factors / genetics
IF 3.193
引用数 2
リソース情報
酵母 FY11372