RRC ID 53203
著者 Funabashi T, Katoh Y, Okazaki M, Sugawa M, Nakayama K.
タイトル Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis.
ジャーナル J Cell Biol
Abstract Intraflagellar transport (IFT) is crucial for the assembly and maintenance of cilia and is mediated by IFT particles containing IFT-A and IFT-B complexes. IFT-B powered by heterotrimeric kinesin-II and IFT-A powered by the dynein-2 complex are responsible for anterograde and retrograde protein trafficking, respectively. However, little is known about the molecular basis of the trafficking of these IFT particles regulated by kinesin and dynein motors. Using the visible immunoprecipitation assay, we identified in this study a three-to-four protein interaction involving the kinesin-II trimer KIF3A-KIF3B-KAP3 and the IFT-B-connecting tetramer IFT38-IFT52-IFT57-IFT88; among the kinesin-II subunits, KIF3B contributed mainly to IFT-B binding. Furthermore, we showed that the ciliogenesis defect of KIF3B-knockout cells can be rescued by the exogenous expression of wild-type KIF3B but not by that of its mutant compromised with respect to IFT-B binding. Thus, interaction of heterotrimeric kinesin-II with the IFT-B-connecting tetramer is crucial for ciliogenesis via the powering of IFT particles to move in the anterograde direction.
巻・号 217(8)
ページ 2867-2876
公開日 2018-8-6
DOI 10.1083/jcb.201801039
PII jcb.201801039
PMID 29903877
PMC PMC6080941
MeSH Adaptor Proteins, Signal Transducing / chemistry Adaptor Proteins, Signal Transducing / metabolism Cells, Cultured Cilia / metabolism Cilia / physiology* Cytoskeletal Proteins / chemistry Cytoskeletal Proteins / metabolism Flagella / metabolism* Gene Knockout Techniques Humans Immunoprecipitation Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Kinesins / physiology Models, Molecular Protein Multimerization Protein Transport Signal Transduction
IF 8.811
リソース情報
遺伝子材料 pCAG2-EGFP-C-KAP3 (RDB20237) pCAG2-EGFP-C-KIF3A (RDB20238) Genome Network Project Human cDNA IRAK106P17 (HGY042777) IRAK034F02 (HGY013722).