RRC ID 53254
著者 Shen Y, Ng LF, Low NP, Hagen T, Gruber J, Inoue T.
タイトル C. elegans miro-1 Mutation Reduces the Amount of Mitochondria and Extends Life Span.
ジャーナル PLoS One
Abstract Mitochondria play a critical role in aging, however, the underlying mechanism is not well understood. We found that a mutation disrupting the C. elegans homolog of Miro GTPase (miro-1) extends life span. This phenotype requires simultaneous loss of miro-1 from multiple tissues including muscles and neurons, and is dependent on daf-16/FOXO. Notably, the amount of mitochondria in the miro-1 mutant is reduced to approximately 50% of the wild-type. Despite this reduction, oxygen consumption is only weakly reduced, suggesting that mitochondria of miro-1 mutants are more active than wild-type mitochondria. The ROS damage is slightly reduced and the mitochondrial unfolded protein response pathway is weakly activated in miro-1 mutants. Unlike previously described long-lived mitochondrial electron transport chain mutants, miro-1 mutants have normal growth rate. These results suggest that the reduction in the amount of mitochondria can affect the life span of an organism through activation of stress pathways.
巻・号 11(4)
ページ e0153233
公開日 2016-1-1
DOI 10.1371/journal.pone.0153233
PII PONE-D-16-07739
PMID 27064409
PMC PMC4827821
MeSH Aging / genetics Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Longevity / genetics* Mitochondria / metabolism Mitochondria / pathology* Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* Mutation / genetics* Oxygen Consumption Phenotype RNA, Messenger / genetics Reactive Oxygen Species / metabolism Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction
IF 2.74
引用数 10
リソース情報
線虫 tm1966