| RRC ID |
53296
|
| Author |
Guan L, Ma X, Zhang J, Liu JJ, Wang Y, Ding M.
|
| Title |
The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions.
|
| Journal |
PLoS Genet
|
| Abstract |
Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 induces the formation of cell protrusions in C. elegans. CHDP-1 is anchored to the cortex through its amphipathic helix. CHDP-1 associates through its CH domain with the small GTPase Rac1/CED-10, which is a key regulator of the actin cytoskeleton. CHDP-1 preferentially binds to the GTP-bound active form of the CED-10 protein and preserves the membrane localization of GTP-CED-10. Hence, by coupling membrane expansion to Rac1-mediated actin dynamics, CHDP-1 promotes the formation of cellular protrusions in vivo.
|
| Volume |
12(7)
|
| Pages |
e1006163
|
| Published |
2016-7-1
|
| DOI |
10.1371/journal.pgen.1006163
|
| PII |
PGENETICS-D-16-00445
|
| PMID |
27415421
|
| PMC |
PMC4944944
|
| MeSH |
Actin Cytoskeleton / metabolism
Actins / metabolism
Alleles
Animals
Animals, Genetically Modified
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism*
Calcium-Binding Proteins / metabolism*
Cell Membrane / metabolism
Cell Movement
Cell Surface Extensions / metabolism*
Gene Expression Regulation*
Microfilament Proteins / metabolism*
Models, Genetic
Monomeric GTP-Binding Proteins / metabolism
Neurons / metabolism
Phenotype
Protein Binding
Sequence Analysis, DNA
Transgenes
rac GTP-Binding Proteins / metabolism*
|
| IF |
5.175
|
| Times Cited |
3
|
| Resource |
| C.elegans |
tm4947 |
