論文 - 詳細
| RRC ID | 53312 |
|---|---|
| 著者 | Ladage ML, King SD, Burks DJ, Quan DL, Garcia AM, Azad RK, Padilla PA. |
| タイトル | Glucose or Altered Ceramide Biosynthesis Mediate Oxygen Deprivation Sensitivity Through Novel Pathways Revealed by Transcriptome Analysis in Caenorhabditis elegans. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
Individuals with type 2 diabetes display metabolic abnormalities, such as hyperglycemia, increased free fatty acids, insulin resistance, and altered ceramide levels, that contribute to vascular dysfunctions and compromised oxygen delivery. Caenorhabditis elegans fed a glucose-supplemented diet or with altered ceramide metabolism, due to a hyl-2 mutation, are sensitive to oxygen deprivation (anoxia). Our experiments showed that the combination of these factors further decreased the anoxia survival. RNA-sequencing analysis was performed to assess how a glucose-supplemented diet and/or a hyl-2 mutation altered the transcriptome. Comparison analysis of transcripts associated with anoxia-sensitive animals [hyl-2(tm2031) mutation or a glucose diet] revealed 199 common transcripts encoded by genes with known or predicted functions involving innate immunity, cuticle function (collagens), or xenobiotic and endobiotic phase I and II detoxification system. Use of RNA interference (RNAi) to target gene products of the xenobiotic and endobiotic phase I and II detoxification system (UDP-glycosyltransferase and Cytochrome p450 genes; ugt-15, ugt-18, ugt-19, ugt-41, ugt-63, cyp-13A12, cyp-25A1, and cyp-33C8) increased anoxia survival in wild-type animals fed a standard diet. Anoxia sensitivity of the hyl-2(tm2031) animals was suppressed by RNAi of cyp-25A1 or cyp-33C8 genes. A glucose diet fed to the P0 hermaphrodite decreased the anoxia survival of its F1 embryos; however, the RNAi of ugt-63 and cyp-33C8 suppressed anoxia sensitivity. These studies provide evidence that the detoxification system impacts oxygen deprivation responses and that C. elegans can be used to model the conserved detoxification system. |
| 巻・号 | 6(10) |
| ページ | 3149-3160 |
| 公開日 | 2016-10-13 |
| DOI | 10.1534/g3.116.031583 |
| PII | g3.116.031583 |
| PMID | 27507791 |
| PMC | PMC5068937 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Ceramides / biosynthesis* Cluster Analysis Computational Biology / methods Gene Expression Profiling* Gene Knockdown Techniques Glucose / biosynthesis* Hypoxia / genetics Hypoxia / metabolism Lipid Metabolism Male Metabolic Detoxication, Phase I / genetics Metabolic Detoxication, Phase II / genetics Mutation Oxygen / metabolism* Phenotype Signal Transduction* Transcriptome* Xenobiotics / metabolism |
| IF | 2.781 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2031 |