論文 - 詳細
| RRC ID | 53319 |
|---|---|
| 著者 | Ossareh-Nazari B, Katsiarimpa A, Merlet J, Pintard L. |
| タイトル | RNAi-Based Suppressor Screens Reveal Genetic Interactions Between the CRL2LRR-1 E3-Ligase and the DNA Replication Machinery in Caenorhabditis elegans. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
Cullin-RING E3-Ligases (CRLs), the largest family of E3 ubiquitin-Ligases, regulate diverse cellular processes by promoting ubiquitination of target proteins. The evolutionarily conserved Leucine Rich Repeat protein 1 (LRR-1) is a substrate-recognition subunit of a CRL2LRR-1 E3-ligase. Here we provide genetic evidence supporting a role of this E3-enzyme in the maintenance of DNA replication integrity in Caenorhabditis elegans Through RNAi-based suppressor screens of lrr-1(0) and cul-2(or209ts) mutants, we identified two genes encoding components of the GINS complex, which is part of the Cdc45-MCM-GINS (CMG) replicative helicase, as well as CDC-7 and MUS-101, which drives the assembly of the CMG helicase during DNA replication. In addition, we identified the core components of the ATR/ATL-1 DNA replication checkpoint pathway (MUS-101, ATL-1, CLSP-1, CHK-1). These results suggest that the CRL2LRR-1 E3-ligase acts to modify or degrade factor(s) that would otherwise misregulate the replisome, eventually leading to the activation of the DNA replication checkpoint. |
| 巻・号 | 6(10) |
| ページ | 3431-3442 |
| 公開日 | 2016-10-13 |
| DOI | 10.1534/g3.116.033043 |
| PII | g3.116.033043 |
| PMID | 27543292 |
| PMC | PMC5068962 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism DNA Replication* Epistasis, Genetic* Gene Expression Regulation* Genetic Testing Multiprotein Complexes / metabolism Mutation Protein Subunits RNA Interference* Receptors, Cytokine / genetics* Receptors, Cytokine / metabolism Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism |
| IF | 2.781 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3543 |