論文 - 詳細
| RRC ID | 53398 |
|---|---|
| 著者 | Peel N, Iyer J, Naik A, Dougherty MP, Decker M, O'Connell KF. |
| タイトル | Protein Phosphatase 1 Down Regulates ZYG-1 Levels to Limit Centriole Duplication. |
| ジャーナル | PLoS Genet |
| Abstract |
In humans perturbations of centriole number are associated with tumorigenesis and microcephaly, therefore appropriate regulation of centriole duplication is critical. The C. elegans homolog of Plk4, ZYG-1, is required for centriole duplication, but our understanding of how ZYG-1 levels are regulated remains incomplete. We have identified the two PP1 orthologs, GSP-1 and GSP-2, and their regulators I-2SZY-2 and SDS-22 as key regulators of ZYG-1 protein levels. We find that down-regulation of PP1 activity either directly, or by mutation of szy-2 or sds-22 can rescue the loss of centriole duplication associated with a zyg-1 hypomorphic allele. Suppression is achieved through an increase in ZYG-1 levels, and our data indicate that PP1 normally regulates ZYG-1 through a post-translational mechanism. While moderate inhibition of PP1 activity can restore centriole duplication to a zyg-1 mutant, strong inhibition of PP1 in a wild-type background leads to centriole amplification via the production of more than one daughter centriole. Our results thus define a new pathway that limits the number of daughter centrioles produced each cycle. |
| 巻・号 | 13(1) |
| ページ | e1006543 |
| 公開日 | 2017-1-1 |
| DOI | 10.1371/journal.pgen.1006543 |
| PII | PGENETICS-D-16-01128 |
| PMID | 28103229 |
| PMC | PMC5289615 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics* Carrier Proteins / metabolism Centrioles / metabolism* Down-Regulation* Mutation Protein Kinases / genetics Protein Kinases / metabolism* Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism* |
| IF | 5.175 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3972 tm5187 tm301 |