論文 - 詳細
| RRC ID | 53407 |
|---|---|
| 著者 | Zhou X, Feng X, Mao H, Li M, Xu F, Hu K, Guang S. |
| タイトル | RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway. |
| ジャーナル | Nat Struct Mol Biol |
| Abstract |
Expression of rRNA affects cell growth and proliferation, but mechanisms that modulate rRNA levels are poorly understood. We conducted a genetic screen for factors that negatively regulate generation of endogenous short interfering RNA (endo-siRNA) in Caenorhabditis elegans and identified a suppressor of siRNA (susi-1) and antisense ribosomal siRNAs (risiRNAs). risiRNAs show sequence complementary to 18S and 26S rRNAs and require RNA-dependent RNA polymerases (RdRPs) for their production. They act through the nuclear RNA interference (RNAi) pathway to downregulate pre-rRNA. Stress stimuli, including low temperature and UV irradiation, induced the accumulation of risiRNAs. SUSI-1 is a homolog of the human DIS3L2 exonuclease involved in 3'-5' degradation of oligouridylated RNAs. In susi-1 mutant and in low temperature-treated animals, 3'-tail oligouridylated 26S rRNA accumulated. The injection of oligouridylated rRNA elicited nuclear accumulation of NRDE-3. Our findings identify a new subset of 22G-RNAs that regulate pre-rRNA expression and a mechanism to maintain rRNA homeostasis. |
| 巻・号 | 24(3) |
| ページ | 258-269 |
| 公開日 | 2017-3-1 |
| DOI | 10.1038/nsmb.3376 |
| PII | nsmb.3376 |
| PMID | 28165511 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Cell Nucleus / metabolism* Cell Nucleus / radiation effects Down-Regulation / genetics Down-Regulation / radiation effects Green Fluorescent Proteins / metabolism High-Throughput Nucleotide Sequencing Models, Biological Mutation / genetics RNA Interference* RNA Precursors / genetics RNA Precursors / metabolism* RNA Stability / radiation effects RNA, Antisense / genetics* RNA, Antisense / metabolism RNA, Ribosomal / genetics RNA, Ribosomal / metabolism* RNA, Small Interfering / metabolism* RNA-Dependent RNA Polymerase / metabolism* Ribosomes / genetics Ribosomes / metabolism* Sequence Analysis, RNA Sequence Homology, Amino Acid Subcellular Fractions / metabolism Subcellular Fractions / radiation effects Temperature Ultraviolet Rays Uridine / metabolism |
| IF | 11.98 |
| 引用数 | 13 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1200 |