RRC ID 53407
Author Zhou X, Feng X, Mao H, Li M, Xu F, Hu K, Guang S.
Title RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway.
Journal Nat Struct Mol Biol
Abstract Expression of rRNA affects cell growth and proliferation, but mechanisms that modulate rRNA levels are poorly understood. We conducted a genetic screen for factors that negatively regulate generation of endogenous short interfering RNA (endo-siRNA) in Caenorhabditis elegans and identified a suppressor of siRNA (susi-1) and antisense ribosomal siRNAs (risiRNAs). risiRNAs show sequence complementary to 18S and 26S rRNAs and require RNA-dependent RNA polymerases (RdRPs) for their production. They act through the nuclear RNA interference (RNAi) pathway to downregulate pre-rRNA. Stress stimuli, including low temperature and UV irradiation, induced the accumulation of risiRNAs. SUSI-1 is a homolog of the human DIS3L2 exonuclease involved in 3'-5' degradation of oligouridylated RNAs. In susi-1 mutant and in low temperature-treated animals, 3'-tail oligouridylated 26S rRNA accumulated. The injection of oligouridylated rRNA elicited nuclear accumulation of NRDE-3. Our findings identify a new subset of 22G-RNAs that regulate pre-rRNA expression and a mechanism to maintain rRNA homeostasis.
Volume 24(3)
Pages 258-269
Published 2017-3-1
DOI 10.1038/nsmb.3376
PII nsmb.3376
PMID 28165511
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Cell Nucleus / metabolism* Cell Nucleus / radiation effects Down-Regulation / genetics Down-Regulation / radiation effects Green Fluorescent Proteins / metabolism High-Throughput Nucleotide Sequencing Models, Biological Mutation / genetics RNA Interference* RNA Precursors / genetics RNA Precursors / metabolism* RNA Stability / radiation effects RNA, Antisense / genetics* RNA, Antisense / metabolism RNA, Ribosomal / genetics RNA, Ribosomal / metabolism* RNA, Small Interfering / metabolism* RNA-Dependent RNA Polymerase / metabolism* Ribosomes / genetics Ribosomes / metabolism* Sequence Analysis, RNA Sequence Homology, Amino Acid Subcellular Fractions / metabolism Subcellular Fractions / radiation effects Temperature Ultraviolet Rays Uridine / metabolism
IF 11.98
Times Cited 13
C.elegans tm1200